Phospho-HSP27 (Ser82) (Clone: CB2) rabbit mAb FITC conjugate
Produktgrößen
10 Tests
£532,00
12-4301-10TESTS
100 Tests
£792,00
12-4301-100TESTS
Über dieses Produkt
- SKU:
- 12-4301
- Zusätzliche Namen:
- Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27; SRP27; HSP28
- translate.label.attr.clone:
- HSP27S82-CB2
- Klonalität:
- Monoclonal
- Konjugat:
- FITC
- Weitere Details:
- Human Hsp27 (also called HspB) is a heat shock protein whose level is upregulated when cells are exposed to conditions that alter protein folding. Hsp27 is an ATP-independent molecular chaperone involved in the protein refolding machinery (1).It functions as a tool to trap and store stress-induced mis-folded polypeptides to avoid their aggregation and to indirectly promote their refolding or proteolytic degradation (2;3). Hsp27 is also known for its control of cytoskeletal organization (4-6) and for its antiapoptotic and antioxidant properties (7-9). Hsp27 phosphorylation level could be modified by agents such as phorbol ester; calcium ionophores; or stress conditions such as heat shock (10). Human Hsp27 contains three phosphorylatable serine sites (Ser15; Ser78; and Ser82) located in its N-terminal domain whereas murine Hsp27; also called Hsp25; has only two phosphorylated serine sites (Ser15 and Ser86). Several kinases phosphorylate Hsp27 (11) including mitogen-activated protein kinases associated protein kinases (MAPK/MK2;3); MK5-PRAK; PKCg; and PKD. Moreover; threonine 143 (Thr143) of human Hsp27 appears to be phosphorylated by cGMP-dependent protein kinase.Therefore; Hsp27 often displays complex patterns of phosphorylation since several signaling pathways; including those induced by stress; mitogen; and differentiation; activate these kinases. Hsp27 is involved with cell differentiation (12). It also modifies apoptotic pathways through upstream and downstream of mitochondria by decreasing activity of caspase 9 and caspase 3 (13;14).These events are activated in heat shock; oxidative stress; and endoplasmic reticulum stress-induced apoptosis (15; 16). Hsp27 Phosphorylation: Recently; it has been shown that Sec6 is involved with Hsp27 Ser78; and Ser82 phosphorylation through p38 MAPK promoting cell migration and inhibition of apoptosis induced by TNFa(17). MAPKAPK2 (MK2)/Hsp27 axis have been shown to promote tumorigenesis through phosphorylation of Hsp27 by MK2 (18). Hsp27 (Ser78/82) phosphorylation through PKCa (Thr638); p38MAPK (Thr180/Tyr182) is elevated by chemokine CC-motif ligand 2 (CCL2) in platelets suggesting that PKCa/pMAPK/Hsp27 axis may be target for antiplatelet drugs and the treatment for cardiovascular diseases (19).
- Format:
- Conjugated
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human phospho Hsp27
- Isotyp:
- Rabbit IgG1k
- Reaktivitäten:
- Human, Mouse
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Monoclonal Antibody
- Datenblatt des Herstellers:1.jpg