Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

Phospho-Btk (Tyr551) (Clone: G12) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4272-20UL
200 µl
£1021,00
12-4272-200UL
Über dieses Produkt
SKU:
12-4272
Zusätzliche Namen:
Bruton tyrosine kinase; Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; AGMX1; B-cell progenitor kinase; BPK
translate.label.attr.clone:
BtkY551-G12
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
Btk is a major node in the B-cell receptor signaling pathway; where it regulates B cell maturation; activation; survival; differentiation; and proliferation. Btk is activated by Src family kinases; including Lyn; which phosphorylates Btk at Tyr551. Upon phosphorylation at this site; Btk is recruited to the plasma membrane where autophosphorylation at Tyr223 occurs. The Btk signaling pathway is a major target of small molecule inhibitors for B-cell lymphoma; autoimmune diseases; and non-Hodgkin's lymphomas. These inhibitors either form a covalent bond at Cys481 in the ATP-binding site or serve as reversible inhibitors that bind the SH3 pocket and stabilize inactive Btk.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr551 of human phospho Btk
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Btk (Y551) antibody BtkY551-G12 or Company B antibody at 0.1 µg/mL (manufacturer's recommended concentration).
Fig-3: Peptide ELISA using BtkY551-G12 titered starting from 0.3 µg/mL shows binding to only Btk pY551 phospho peptide and no cross-reactivity to Btk Y551 non-phospho peptide or to control peptides.
Fig-4: Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.25 mg/mL total protein coated lysate and 1 µg/mL BtkY551-G12 IgG.
Fig-4: Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.25 mg/mL total protein coated lysate and 1 µg/mL BtkY551-G12 IgG.