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Antikörper

Phospho-c-Cbl (Tyr774) (Clone: R3B8) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4250-20UL
200 µl
£1021,00
12-4250-200UL
Über dieses Produkt
SKU:
12-4250
Zusätzliche Namen:
E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; RING-type E3 ubiquitin transferase; Signal transduction protein CBL; CBL2; RNF55
translate.label.attr.clone:
CblY774-R3B8
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
The c-Cbl (Casitas B-lineage Lymphoma) proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that contains multiple functional domains; including an amino-terminal tyrosine kinase-binding (TKB) domain; a RING finger motif; and a proline-rich region. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) and on other nonreceptor tyrosine kinases; while the RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (1). The proline-rich region contains 14-3-3 protein-binding and SH3 domain-binding motifs. c-Cbl is phosphorylated at Y700; Y731; and Y774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for immunoglobulins; antigens; hormones; growth factors; and cytokines. Phosphorylated Y774 interacts with the SH2 domain of Crk (1;2). The c-Cbl adapter protein is expressed in the cytoplasm in all tissues; with especially high levels of expression in hematopoietic cells (3;4). Through its many functional sites; c-Cbl plays key roles in the positive and negative regulation of vital cell functions; including T Cell Receptor-mediated cellular immune responses. In human cancer tissues; c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5).
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human phospho c-Cbl
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-c-Cbl (Tyr774) antibody CblY774-R3B8 at 0.1 µg/mL.
Fig-3: Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (gray) or treated with IFNa + IL-4 + pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or treated (green) using Phospho-c-Cbl (Tyr774) antibody CblY774-R3B8 at 0.01 µg/mL.
Fig-3: Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (gray) or treated with IFNa + IL-4 + pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or treated (green) using Phospho-c-Cbl (Tyr774) antibody CblY774-R3B8 at 0.01 µg/mL.