Phospho-KSR1 (Ser392) (Clone: 3A4) rabbit mAb SureLight conjugate
Produktgrößen
10 Tests
£532,00
12-4201-10TESTS
100 Tests
£792,00
12-4201-100TESTS
Über dieses Produkt
- SKU:
- 12-4201
- Zusätzliche Namen:
- Kinase suppressor of Ras 1
- translate.label.attr.clone:
- KSR1S392-3A4
- Klonalität:
- Monoclonal
- Konjugat:
- SureLight 488
- Weitere Details:
- The kinase suppressor of Ras 1 (KSR1) is a molecular scaffold protein that regulates the activation of the Raf/MEK/extracellular signal-regulated kinase (ERK) signal transduction pathway (1-3). KSR1 expression regulates the intensity and duration of growth factor-induced ERK activation to modulate cell proliferation; H-RasV12-induced transformation and senescence; and adipogenic potential. KSR1 disruption in mammalian models inhibits oncogenic Ras-induced senescence and transformation in vitro and in vivo (4;5). KSR1 translocates to the plasma membrane in response to growth factor treatment and Ras activation; where it forms a complex involving Raf-1; MEK1 and 14-3-3 protein and facilitates the activation of MEK by Raf and of ERK by MEK (6). KSR1 also mediates ERK-dependent negative feedback signaling on Raf; MEK; and KSR1 (7). In non-stimulated cells; KSR1 is sequestered in the cytosol through 14-3-3 protein binding after phosphorylation by C-TAK1 at Ser297 and Ser392. Meanwhile; KSR1 constitutively interacts with MEK and ERK. Upon growth factor stimulation; activated Ras triggers the dephosphorylation of KSR1 at Ser392 by PP2A; leading to the release of 14-3-3 protein from its binding sites. This in turn allows phospho KSR1 to translocate to the cell membrane; where phospho KSR1 forms a complex with Raf; MEK and ERK (8). KSR1 thus potentially enhances the phosphorylation of Raf; MEK and ERK; facilitating the upstream signalling transduction as well as regulating multiple cellular functions by activation of various substrates.
- Format:
- Conjugated
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Ser392 of human phospho KSR1
- Isotyp:
- Rabbit IgG1k
- Reaktivitäten:
- Human, Mouse
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Monoclonal Antibody
- Datenblatt des Herstellers:1.jpg