Phospho-CREB (Ser133) (Clone: 4D11) rabbit mAb PE conjugate
Produktgrößen
10 Tests
£532,00
12-4174-10TESTS
100 Tests
£792,00
12-4174-100TESTS
Über dieses Produkt
- SKU:
- 12-4174
- Zusätzliche Namen:
- Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
- translate.label.attr.clone:
- CREBS133-4D11
- Klonalität:
- Monoclonal
- Konjugat:
- PE
- Weitere Details:
- The cyclic AMP-responsive element (CRE)-binding protein (CREB) is a 43-kDa leucine zipper transcription factor that belongs to the CREB/ATF family and modulates the activity of many eukaryotic transcription factors by altering their subcellular localization; DNA binding; or transactivation response. It regulate downstream targets involving in various cellular functions including cell proliferation; survival; and differentiation (1-2). CREB activity is considered critical for neuronal function and regulates synaptic plasticity; long-term memory formation and neuronal survival (3). CREB serine 133 (Ser-133) residue is phosphorylated by various kinases and this phosphorylation promotes the interaction of CREB with a number of transcription coactivators; especially the histone acetyltransferases CREB-binding protein (CBP) or p300 (4;5). CREB can be phosphorylated and thus activated in response to various stimuli such as growth factors; neurotransmitters; stress signals that increase intracellular cAMP or calcium levels. CREB is also activated by phosphorylation at Ser-133 through nuclear translocation of transducer of regulated CREB activity (TORC) coactivators (6;7). CREB is phosphorylated in response to hormonal stimuli that increase intracellular cAMP production; but it can also be phosphorylated in response to a wide variety of extra-cellular signals; including growth factors; osmotic stress and ultraviolet irradiation. Several kinases including ribosomal protein S6 kinase (pp90RSK); protein kinase C (PKC); protein kinase B/AKT; and mitogen- and stress-activated protein kinase (MSK-1) can phosphorylate CREB at Ser-133 (1;8). Different growth factors such as mast/stem cell growth factor; basic fibroblast growth factor; and Granulocyte-macrophage colony-stimulating factor (GM-CSF); can all induce phosphorylation of CREB (9; 10). Phospho CREB; upon activation; dimerizes and binds to the promoter regions of its target gene that contains cAMP response element (CRE site); TGACGTCA; or CRE half sites CGTCA/TGACG; and promotes the recruitment of its transcriptional coactivators; CBP/p300; for CREB-mediated transcription. Therefore; phospho CREB can regulate various cellular mechanisms through modulating its target genes. CREB is progressively dephosphorylated by the Ser/Thr phosphatases protein phosphatase 1 (PP1) and PP2A.
- Format:
- Conjugated
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human phospho CREB
- Isotyp:
- Rabbit IgG1k
- Reaktivitäten:
- Human, Mouse
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Monoclonal Antibody
- Datenblatt des Herstellers:1.jpg