Phospho-Ask1 (Ser83) (Clone: G4) rabbit mAb APC conjugate
Produktgrößen
10 Tests
£532,00
12-4165-10TESTS
100 Tests
£792,00
12-4165-100TESTS
Über dieses Produkt
- SKU:
- 12-4165
- Zusätzliche Namen:
- Apoptosis signal-regulating kinase 1; Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MAP3K5; MAPKKK5; MEKK5
- translate.label.attr.clone:
- Ask1S83-G4
- Klonalität:
- Monoclonal
- Konjugat:
- APC
- Weitere Details:
- Stress signaling in cells are partly mediated through c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). A kinase upstream of these stress-mediated pathways is apoptosis signal-regulating kinase 1 (ASK1). ASK1 is a serine/threonine kinase that belongs to the family of mitogen-activated protein kinase kinase kinase (MAP3K). It is endogenously expressed in various cells. Various stress signaling activates ASK1 including reactive oxygen species (ROS); tumor necrosis factor alpha (TNFa); lipopolysaccharide (LPS) and endoplasmic reticulum (ER) stress. These events ultimately lead to the activation of JNK and p38 MAPK. As a result; ASK1-MAPK signaling is associated with various cellular stress responses including apoptosis; differentiation; and inflammation. Several studies show that aberrant ASK1 signaling leads to severe human diseases such as neurodegenerative diseases; induction of inflammatory diseases and cancer. The phosphorylation of the threonine residues 838 (Thr838) in human and Thr845 in mice is important for ASK1 activation. At basal inactive state; ASK1 is a homooligomer; which binds to another ASK1 via its C-terminal coiled-coil domain. The N-terminal coiled-coil of ASK1 binds to thioredoxin (Trx); which suppresses ASK1 kinase activity. Under oxidative stress; oxidized Trx is separated from ASK1 and unbond ASK1 is activated by phosphorylation. Calcium influx and oxidative stress can elicit phosphorylation of the ASK1. After H2O2 injury; tumor necrosis factor 2; (TRAF2) and TRAF6 act as positive regulator of ASK1. 14-3-3 proteins; act as negative regulator of ASK1 by binding to the C-terminal of ASK1 after Ser966 phosphorylation. However oxidative stress promotes dephosphorylation of Ser966 and lead to detachment of 14-3-3. TNF and Fas death receptor activate ASK1. ASK1 is an early signal stress responder to oxidative stress in cerebral ischemia. ASK1 has also been implicated in thrombosis; brain edema; inflammatory response; and reactive gliosis.
- Format:
- Conjugated
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1
- Isotyp:
- Rabbit IgG1k
- Reaktivitäten:
- Human, Mouse, Rat
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Monoclonal Antibody
- Datenblatt des Herstellers:1.jpg