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Antikörper

Phospho-KSR1 (Ser392) (Clone: 3A4) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4092-20UL
200 µl
£1021,00
12-4092-200UL
Über dieses Produkt
SKU:
12-4092
Zusätzliche Namen:
Kinase suppressor of Ras 1
translate.label.attr.clone:
KSR1S392-3A4
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
The kinase suppressor of Ras 1 (KSR1) is a molecular scaffold protein that regulates the activation of the Raf/MEK/extracellular signal-regulated kinase (ERK) signal transduction pathway (1-3). KSR1 expression regulates the intensity and duration of growth factor-induced ERK activation to modulate cell proliferation; H-RasV12-induced transformation and senescence; and adipogenic potential. KSR1 disruption in mammalian models inhibits oncogenic Ras-induced senescence and transformation in vitro and in vivo (4;5). KSR1 translocates to the plasma membrane in response to growth factor treatment and Ras activation; where it forms a complex involving Raf-1; MEK1 and 14-3-3 protein and facilitates the activation of MEK by Raf and of ERK by MEK (6). KSR1 also mediates ERK-dependent negative feedback signaling on Raf; MEK; and KSR1 (7). In non-stimulated cells; KSR1 is sequestered in the cytosol through 14-3-3 protein binding after phosphorylation by C-TAK1 at Ser297 and Ser392. Meanwhile; KSR1 constitutively interacts with MEK and ERK. Upon growth factor stimulation; activated Ras triggers the dephosphorylation of KSR1 at Ser392 by PP2A; leading to the release of 14-3-3 protein from its binding sites. This in turn allows phospho KSR1 to translocate to the cell membrane; where phospho KSR1 forms a complex with Raf; MEK and ERK (8). KSR1 thus potentially enhances the phosphorylation of Raf; MEK and ERK; facilitating the upstream signalling transduction as well as regulating multiple cellular functions by activation of various substrates.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser392 of human phospho KSR1
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : KSR1S392-3A4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of Phospho-KSR1(S392) antibody KSR1S392-3A4 (green).
Fig-3: Flow cytometric analysis of HeLa human adenocarcinoma cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using Phospho-KSR1 (S392) antibody; KSR1S392-3A4 at 0.1 µg/mL.
Fig-3: Flow cytometric analysis of HeLa human adenocarcinoma cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using Phospho-KSR1 (S392) antibody; KSR1S392-3A4 at 0.1 µg/mL.