Phospho-Chk1 (Ser317) (Clone: F10) rabbit mAb
Produktgrößen
20 µl
£631,00
12-4087-20UL
200 µl
£1021,00
12-4087-200UL
Über dieses Produkt
- SKU:
- 12-4087
- Zusätzliche Namen:
- Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1
- translate.label.attr.clone:
- Chk1S317-F10
- Klonalität:
- Monoclonal
- Konjugat:
- Unconjugated
- Weitere Details:
- The act of DNA damaged response and cell cycle checkpoints requires the activation of four protein kinases that form the canonical ATR-Chk1 and ATM-Chk2 pathways. ATR activation requires the generation of structures containing single strand DNA (ssDNA) adjacent to double strand DNA (dsDNA). Such ssDNA is coated with replication protein A complex and attracts ATR (1;2). The accumulation of ATR to damage sites results in initial activation of ATR. ATR phosphorylates proteins at the ssDNA which are called checkpoint regulators. The accumulation and phosphorylation of these checkpoint regulators further stimulates the catalytic activity of ATR. ATR-induced Chk1 phosphorylation likely occurs at the sites of DNA damage on chromatin (3-5). The activated ATR phosphorylates Chk1 at Ser317 and Ser345 in its C-terminal regulatory domain. Phospho Chk1 is critical for DNA damage checkpoint activation; replication control; and cell viability (6-8). Functionally; ATR-mediated phosphorylation elevates phospho Chk1 catalytic activity. The N-terminal catalytic domain of Chk1 adopts an open kinase conformation and the deletion of C-terminal domain increases Chk1 catalytic activity.
- Format:
- Purified
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Ser317 of human phospho Chk1
- Isotyp:
- Rabbit IgG1k
- Reaktivitäten:
- Human, Mouse
- Versandbedingungen:
- Blue Ice
- Lagerbedingungen:
- Store at -20[o]C. Avoid repeated freeze and thaw cycles.
- Hersteller:
- Abeomics
- Typ:
- Antibodies:Monoclonal Antibody
- Datenblatt des Herstellers:1.jpg
