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Antikörper

Phospho-CREB (Ser133) (Clone: 4D11) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4081-20UL
200 µl
£1021,00
12-4081-200UL
Über dieses Produkt
SKU:
12-4081
Zusätzliche Namen:
Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
translate.label.attr.clone:
CREBS133-4D11
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
The cyclic AMP-responsive element (CRE)-binding protein (CREB) is a 43-kDa leucine zipper transcription factor that belongs to the CREB/ATF family and modulates the activity of many eukaryotic transcription factors by altering their subcellular localization; DNA binding; or transactivation response. It regulate downstream targets involving in various cellular functions including cell proliferation; survival; and differentiation (1-2). CREB activity is considered critical for neuronal function and regulates synaptic plasticity; long-term memory formation and neuronal survival (3). CREB serine 133 (Ser-133) residue is phosphorylated by various kinases and this phosphorylation promotes the interaction of CREB with a number of transcription coactivators; especially the histone acetyltransferases CREB-binding protein (CBP) or p300 (4;5). CREB can be phosphorylated and thus activated in response to various stimuli such as growth factors; neurotransmitters; stress signals that increase intracellular cAMP or calcium levels. CREB is also activated by phosphorylation at Ser-133 through nuclear translocation of transducer of regulated CREB activity (TORC) coactivators (6;7). CREB is phosphorylated in response to hormonal stimuli that increase intracellular cAMP production; but it can also be phosphorylated in response to a wide variety of extra-cellular signals; including growth factors; osmotic stress and ultraviolet irradiation. Several kinases including ribosomal protein S6 kinase (pp90RSK); protein kinase C (PKC); protein kinase B/AKT; and mitogen- and stress-activated protein kinase (MSK-1) can phosphorylate CREB at Ser-133 (1;8). Different growth factors such as mast/stem cell growth factor; basic fibroblast growth factor; and Granulocyte-macrophage colony-stimulating factor (GM-CSF); can all induce phosphorylation of CREB (9; 10). Phospho CREB; upon activation; dimerizes and binds to the promoter regions of its target gene that contains cAMP response element (CRE site); TGACGTCA; or CRE half sites CGTCA/TGACG; and promotes the recruitment of its transcriptional coactivators; CBP/p300; for CREB-mediated transcription. Therefore; phospho CREB can regulate various cellular mechanisms through modulating its target genes. CREB is progressively dephosphorylated by the Ser/Thr phosphatases protein phosphatase 1 (PP1) and PP2A.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human phospho CREB
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with calyculin A (green) using 1ng/mL Phospho-CREB (Ser133) antibody CREBS133-4D11.
Fig-3: Western blot analysis of SK.N.MC cell extract untreated or treated with forskolin and IBMX using 0.1µg/mL Phospho-CREB (Ser133) antibody CREBS133-4D11.
Fig-4: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with PDGF (green) using Phospho-CREB (Ser133) antibody CREBS133-4D11 at 0.1µg/mL.
Fig-4: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with PDGF (green) using Phospho-CREB (Ser133) antibody CREBS133-4D11 at 0.1µg/mL.
Fig-5: Flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (blue) or untreated (red) or treated with forskolin (green) using 0.01 µg/mL of Phospho-CREB (Ser133) antibody CREBS133-4D11 or Company C antibody at 0.02 µg/mL (manufacturer's recommended concentration).