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Antikörper

Phospho-Chk2 (Thr68) (Clone: D12) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4080-20UL
200 µl
£1021,00
12-4080-200UL
Über dieses Produkt
SKU:
12-4080
Zusätzliche Namen:
Serine/threonine-protein kinase Chk2; Cds1 homolog; Hucds2; CHEK2; RAD53
translate.label.attr.clone:
Chk2T68-D12
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
Checkpoint kinase 2 (Chk2) plays a major role in the checkpoint response to DNA damage. Chk2 is initially inactive in its monomeric; unphosphorylated form. Phosphorylation at Thr68 induces homodimerization; initiating autophosphorylation within the kinase loop at Ser516 and phorphorylation events within the auto-inhibitory loop at Thr383 and Thr387. After these phosphorylations; active dimers and monomers can then phosphorylate substrates such as Cdc25C and BRCA1. In humans; Chk2 genetic deletion and missense variants have been found to be associated with increased risk of breast and colon cancer. Constitutively phosphorylated Chk2 at Thr68 has been found in many human cancer cell lines; especially ones with mutations in p53.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human phospho Chk2
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human, Mouse
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Western blot analysis of HEK293 cell extract untreated or treated with UV using Phospho Chk2(T68) antibody Chk2T68-D12.
Fig-3: Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or untreated (red) or UV/TPA-treated (green) or untreated and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-Chk2 (T68) antibody Chk2T68-D12 0.1µg/mL.
Fig-4: Chk2T68-D12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of Phospho-Chk2 (T68) antibody Chk2T68-D12 (green).
Fig-4: Chk2T68-D12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of Phospho-Chk2 (T68) antibody Chk2T68-D12 (green).
Fig-5: Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 µg/mL Phospho-Chk2 (T68) antibody Chk2T68-D12 or Company C antibody at 2.3 µg/mL (manufacturer's recommended concentration).