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Antikörper

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Clone: A11) rabbit mAb

Produktgrößen
20 µl
£631,00
12-4005-20UL
200 µl
£1021,00
12-4005-200UL
Über dieses Produkt
SKU:
12-4005
Zusätzliche Namen:
Mitogen-activated protein kinase 3; MAPK3; ERK2; p44-MAPK; PRKM3; Mitogen-activated protein kinase 1; MAPK1; ERK1; p42-MAPK; PRKM1; PRKM2
translate.label.attr.clone:
ERK12T202Y204-A11
Klonalität:
Monoclonal
Konjugat:
Unconjugated
Weitere Details:
Human Erk1 and Erk2 Ser/Thr kinases share 84% sequence identity and nearly all functions. These MAP kinases are activated in response to mitogens and growth factors as part of the Ras-Raf-MEK-ERK signal transduction cascade. This pathway regulates cell survival; differentiation; adhesion; cell cycle progression; and many other cellular processes. Upon phosphorylation; Erk1/2 translocate to the nucleus to activate transcription factors including c-Fos; Elk1; Ets1; and SP-1. There are more than 175 known cytoplasmic and nuclear substrates of Erk1/2. The Erk1/2 cascade is upregulated in many human cancers; even when oncogenic mutations are not found. Multiple small-molecule inhibitors of Erk1/2 have been developed; including ones targeting the ATP-binding site either competitively or irreversibly.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2.
Isotyp:
Rabbit IgG1k
Reaktivitäten:
Human
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Hersteller:
Abeomics
Typ:
Antibodies:Monoclonal Antibody
  • Datenblatt des Herstellers:1.jpg
Fig 2 : Western blot analysis of Ramos cell extract untreated or treated with U0126 followed by no treatment or treatment with PMA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11.
Fig-3: Western blot analysis of 293T cell extract treated with U0126 or TPA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 at 0.1 µg/mL.
Fig-4: ERK12T202Y204-A11 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (Cat# 12-4086) (orange) or of Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 (green)
Fig-4: ERK12T202Y204-A11 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (Cat# 12-4086) (orange) or of Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 (green)
Fig-5: Flow cytometric analysis of Jurkat cells; secondary antibody only negative control (blue); or treated with U0126 (red) or with PMA (green) using Phospho-Erk1/2 (T202/Y204) antibody ERK12T202Y204-A11 or Company C antibody at 0.43 µg/mL (manufacturer's recommended concentration).