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Antikörper

Rabbit anti-human IgG1 FC (DLE mutation) Specific Monoclonal Antibody, Clone 4B6

Produktgrößen
100 ug
£451,00
YR0511-100UG
1 mg
£3131,00
YR0511-1MG
5 mg
£14006,00
YR0511-5MG
10 mg
£23769,00
YR0511-10MG
Über dieses Produkt
SKU:
YR0511
Anwendung:
ELISA, Surface Plasmon Resonance
Klonalität:
Monoclonal
Weitere Details:
The DLE mutation (Ser239Asp/Ile332Glu/Ala330Leu) in the IgG Fc region is a crucial modification in antibody engineering designed to optimize antibody effector functions and stability. This mutation enhances the binding to FcG GammaRIIIa by introducing additional hydrogen bonds that separate the CH2 domain of the Fc segment. Consequently, it significantly boosts antibody-dependent cell-mediated cytotoxicity (ADCC), bolstering the cytotoxic effect of natural killer (NK) cells on target cells. The Ala330Leu substitution within the DLE mutation reduces binding to complement protein C1q, thereby diminishing complement-dependent cytotoxicity (CDC) mediated by the antibody, which helps mitigate non-specific inflammatory responses. Introducing the DLE mutation into the antibody's heavy chain also markedly enhances its stability and solubility. This mutation has been successfully applied in optimizing various therapeutic antibodies, markedly improving their efficacy and stability.
Formulierung:
Purified from cell culture supernatant in an animal-free facility
Isotyp:
IgG1
Molekulargewicht:
150kDa
Reinheit:
>95%
Versandbedingungen:
Blue Ice
Lagerbedingungen:
2-8[o]C Avoid freeze/thaw cycles., -70[o]C Avoid freeze/thaw cycles.
Hersteller:
ABclonal Technology
Typ:
Antibody: Secondary Antibodies
  • Datenblatt des Herstellers:YR0511