Absolute Biotech Company that includes Absolute Antibody and LSBio, have both been extensively involved in being part of the Animal Free antibody movement, and have developed alternatives to offering Recombinant Antibodies and Proteins that require no immunisation of animals. The platform is based on transient, chemical transfection of HEK293 cell lines cultured in a serum free medium. No viral transfection approaches are used.
Through their recombinant technology they ensure reproducibility, defined conformation knowing what is in the vial, high purity as they use Serum-free mammalian expression system, and a large variety of antibody formats can be produced.
Absolute Antibody Reagents LSBio Animal-Free Proteins
Cape Biologix Technologies uses plant-based expression platforms to develop recombinant proteins. A unique alternative where they sequence the DNA digitally and then synthesise it into a physical DNA by using a high expressing PCBP plant expression vectors. The expression vectors are then inserted into Agrobacterium Bacterium. Plants are infiltrated with a solution of transformed bacteria, allowing the delivery of vectors into the cells of plants. A week after infiltration, the leaves of the infected plants are harvest for protein extraction and purification, further they are subjected to stringent quality testing to ensure purity, functionality, and performance.
Using these expression systems for recombinant proteins, they provide wider application capability such as Cell assay, Binding assay/Protein-protein interaction, Cell culture-serum-free medium, Drug related studies, Enzymatic activity in vitro, ELISA standard and its raw materials, In vivo study, Mass spectrometry standard, Protein chip, SDS-PAGE control, Protein structure analysis (Crystal/ electron microscope). Also, to enhance results analysis and research efficiency, they provide multiple tags that can be customised as per request.