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GenomONE™-Neo

HVJ-E Membrane Fusion Transfection Kit

Background

Not just another cationic lipid, GenomONE-Neo is a totally unique alternative to common tranfection reagents.

GenomONE-Neo utilizes the high membrane fusion potential of inactivated Sendai virus particle (Hemagglutinating virus of Japan envelope, HVJ-E) for efficient transfection of nucleic acids, proteins, and small molecules.

The HVJ envelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope.

GenomONE provides ready-to-use kits containing the HVJ envelope and auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer).

Principle

What is HVJ Envelope (HVJ-E)?
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ*). It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.

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It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid at the terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (component of envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potential in humans or animals. HVJ-E can be used safely in standard laboratories, without requiring special procedures or facilities.
*HVJ:Hemagglutinating Virus of Japan.

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Conventional non-viral transfection tools, including cationic lipids, are incorporated into cells through endocytosis which results in degradation of the transferred DNA by lysosomes. On the other hand, HVJ Envelope VECTOR resists degradation by lysosomes, making it easy to transfer the specified DNA. Therefore, HVJ Envelope VECTOR yields highly efficient gene expression.

Sialic acid receptors, which are needed to trigger binding with HN protein, exist in almost all animal cells. Thus, HVJ Envelope VECTOR is useful for a wide range of targets.

  

Features and Advantages

1. Wide usability
GenomONE HVJ Envelope VECTOR KIT is a highly flexible tool for transfecting a wide variety of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cultured cells (adherent and non-adherent) and tissue. GenomONE is useful for transfecting sensitive primary cells and is further distinguished by its ability to deliver contents into live animals. Many literature citations are available for each GenomONE-Neo application.

2. Safety
Unlike cationic lipids,GenomONE-Neo delivers the molecule of interest into cells via membrane fusion, not by endocytosis where cargo may be degraded by lysosomal enzymes. Since GenomeONE-Neo is a purified HVJ (Sendai virus) product, prepared from virus particles completely inactivated for infectious ability and proliferative potential it is completely safe to use without special precautions.

3. Easy
GenomONE provides ready-to-use kits containing the HVJ envelope and required auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, and buffer). Suggested protocols for all major applications are included.

GenomONE-Neo - unique, exciting transfection kit for cells, tissue, and live animals.

Use GenomONE-Neo: for:

  • siRNA - strong knockdowns with low pM amounts of siRNA. Chemical modification of siRNA not required; means high functional expression..
  • DNA - small and large, linear or circular
  • Oligonucleotides - DNA, RNA
  • Proteins - enzymes and antibodies retain function and specificity
  • In vivo transfection - protocol and literature citations for effectiveness of molecules transfected into live animals following direct injection of HVJ-E vector.
  • High throughput screening - transfect multiple molecules simultaneously

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