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Back to Work Essentials

We at 2BScientific provide a wide array of your lab consumables. And if we don`t provide something you would like to try then just make use of our 2BFound service. Where we shall act as an intermediary for you to purchase anything you may need.

Glycomatrix™

Is one of the largest manufacturers of native lectins and lectin conjugates with over 50 years of technical expertise in glycobiology and protein chromatography. Our affinity purified Affypure™ Lectins are freshly conjugated with various fluorophores, biotin, nano-gold and enzymes for IHC applications.

Various lectin-conjugated Separopore® (agarose beads) allow purification of specific biomarkers from various body fluids or cell extracts for glycomics analysis. Glycan-specific multi-lectin kits conjugated with specific fluorophore/biotin/enzyme for the detection of various biomarkers are available for IHC applications.

Diluents & Stabilisers

Ready-to-use sample diluents and robust stabilisers that demonstrate superior stabilisation of proteins conjugated to Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). These stabilisers prevent loss of enzyme activity and maintain the protein's molecular conformation.

The following table will guide you to the optimal choice of our different solutions. D:Diluent, S:Stabiliser

Buffer Standards & Controls Samples HRP-conjugates AP-Conjugates Antibodies
HRP-StabilPLUS**     D/S    
AP-StabilPLUS       D/S  
UNI-StabilPLUS     D/S D/S  
Protein-StabilPLUS D/S       D/S
Antibody Enhancer**     D/S D/S D/S
Sample X-tra D D      
Effect Diluent   D D D D
MultiBOOSTER D D D/S D/S D

*Not an ECO-TEK product
** Cannot be used with antibodies against rabbit immunoglobulin 

BCIP/NBT
Liquid ready-to-use substrate is a highly active and stable formulation utilized for colorimetric detection of Alkaline Phosphatase (AP) activity in membrane assays. Positive reactions form an intense blue/purple precipitate at the site of the reaction. The colour develops when AP catalyzes the dephosphorylation of BCIP and converts NBT to insoluble blue/purple NBT formazan. The intense blue/purple precipitate is very stable and resists fading when exposed to light.

TMB Blotting
Is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue colour during the enzymatic degradation of hydrogen peroxide by HRP. TMB Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site. The substrate does not contain NMP (1-methyl-2pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.

TMB-D Blotting
Is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue colour during the enzymatic degradation of hydrogen peroxide by HRP. TMB-D Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site. The substrate does not contain NMP (1-methyl2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.

Precast Gels

GenScript SurePAGE Bis-Tris gels are high-performance precast mini polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. Sure Page gels are cast in a neutral pH buffer that minimise polyacrylamide hydrolysis and increases gel stability. They run at neutral pH which minimises protein modification compared to Tris-glycine gels.

% Acrylamide Wells No. Well volume Quantity Cat. No.
4-12% 10, 12, 15 80, 60, 40ul 10 gels M00652, M00653, M00654
4-20% 10, 12, 15 80, 60, 40ul 10 gels M00655, M00656, M00657
8-16% 10, 12, 15 80, 60, 40ul 10 gels M00658, M00659, M00660
8% 10, 12, 15 80, 60, 40ul 10 gels M00661, M00662, M00663
10% 10, 12, 15 80, 60, 40ul 10 gels M00664, M00665, M00666
12% 10, 12, 15 80, 60, 40ul 10 gels M00667, M00668, M00669

Compatible Gel Tanks

  • Bio-Rad Mini-PROTEAN[R] II & 3*
  • Bio-Rad Mini-PROTEAN[R] Tetra System*
  • Invitrogen Novex XCell I, II & Surelock[R] (use GenScript Gel Tank Adapter Plates)
  • LONZA PAGEr[R] Minigel Chamber
  • Hoefer Mighty Small (SE 260/SE 250)
  • Hoefer Tall Mighty Small (SE 280)

ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit

Genscript ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit is designed to be a quantitative In Vitro end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices. This method utilizes a modified Limulus Amebocyte Lysate and a synthetic colour producing substrate to detect endotoxin chromogenically in a broad range of 0.01 - 1 EU/ml. In addition, any sample with colour (e.g. cell bacterial culture medium, serum or blood etc.) can not be assayed by this kit.

Key Features
Highly Sensitive: Detect endotoxin concentration in the range of 0.01 - 1 EU/ml
Fast: The incubation period can be shortened to 14 minutes
Reliable: Colour-stabilizer ensures accurate results
Ready-to-use: Kit includes endotoxin-free tips and tubes, LAL reagent water and incubation rack
Broad application: Quantitative in vitro end-point endotoxin test

Plastics

Mobitec's mini columns can function as both spin and affinity chromatography columns. Fill them with the matrix of your choice, or order them pre-filled with, e.g., Sephadex® G-50 resin, different Sephacryl® resins, or silica-based Ni-IDA resin. Filters of different sizes and Luer adapters are offered to customize the columns to your specific needs.

Mini Columns (up to 800 µl) - Mini columns can be used to make your own spin or gravity flow columns with the matrix of your choice. They are also available pre-filled.

  • Fast purification of biomolecules like proteins, antibodies, nucleic acids, etc.
  • Useful for size exclusion or affinity chromatography
  • Ideal for small sample volumes
  • Luer-lock cap is compatible to Luer syringes
  • Filters of different sizes and luer adapters are offered to customize the columns to your specific needs
  • Microfuge-compatible

Empty Mini Columns

Pre-filled Mini Columns

  • MobiSpin Columns for Nucleic Acid Purification
  • Desalting MobiSpin Columns – Separation of Small Contaminants
  • Ni-IDA Columns for Purification of His-tagged Proteins

As always we are here for you, so if there is something we have and you would like more information or a bulk price, just contact us at info@2BScientific.com 

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