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Click here to see the COVID-19 Schematic with the targets involved in each step

Antibodies

Catalogue No. Name Application/Details Specificity
MAB12422-100 Mouse Anti SARS-CoV-2 Spike (S1) Antibody (CR3022), Human IgG1 The original monoclonal antibody was generated by collecting peripheral blood lymphocytes of a patient exposed to the SARS-CoV. Target Antigen: SARS-CoV-2 (COVID-19) and SARS Coronavirus.
MAB12423-50 Mouse Anti SARS-CoV-2 Spike (S1) Antibody (CR3022), Human IgM The original monoclonal antibody was generated by collecting peripheral blood lymphocytes of a patient exposed to the SARS-CoV. Target Antigen: Spike protein S1
200-401-A50 Rabbit anti-Sars Nucleocapsid Protein Antibody [Polyclonal] ELISA : 1:10,000 - 1:50,000
IF Microscopy : User Optimized
Western Blot : 1:2,000 - 1:10,000
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein.  As seen in Nature Research Journal
HM1056 Coronavirus (MERS & SARS-CoV NP) Antibody ELISA, IFA MERS & SARS Coronavirus Nucleoprotein (NP)
HM1059L Coronavirus (SARS-CoV E2) Antibody ELISA, WB Human Coronavirus
HM1060L Coronavirus (SARS-CoV Membrane) Antibody ELISA, WB Human Coronavirus
HM1054 Coronavirus (SARS-CoV NP) Antibody ELISA, WB, IFA SARS Coronavirus Nucleoprotein (NP)
100531 Anti-hCoV OC43 3504 SPTN-5   Antibody recognizes human coronaviruses OC43 nucleoprotein and HKU1
ARG41099 Anti-ACE2 antibody IHC-P, IP, WB Human, Mouse, Rat
ARG10284 Anti-Coronavirus antibody [Clone 5A4] ELISA  
ARG11092 Anti-SARS-CoV Spike protein antibody [1A9] Western Blot, Flow Cytometry reacts to spike proteins from SARS-CoV, SARS-CoV-2 (2019-nCoV), Civet SARS-CoV SZ3 strain, bat SL-CoV Rp3 and Rf1

Antigens

Catalogue No. Name Application Comments
3620-1 Peptide pool for quantification of cytokine secreting cells specific for SARS-CoV-2 S2 and N proteins. Recommended for quantification of the number of cytokine secreting T cells specific for SARS-Cov-S2 and N proteins with ELISpot/Fluorospot. The SARS-CoV-2 derived T-cell epitopes were defined with the help of T-cell assays or from positive MHC binding assays (Ahmed SF., et al.). Based on these assays this peptide pool covers the following MHC alleles: HLA-A*01:01, HLA-A*02:01, HLA-A*03:01, HLA-A*11:01, HLA-A*23:01, HLA-A*24:02, HLA-A*31:01, HLA-A*68:01, HLA-B*07:02, HLA-B*15:01, HLA-B*27:05, HLA-B*35:01, HLA-B*40, HLA-B*51:01, HLA-DRB1*01:01, and HLA-DRB1*04:01.
HP811-60 NEW! Recombinant COVID-19 spike antigen (RBD) Suitable to use in ELISA, CLIA, CMIA and Rapid Test (POCT). Suggested indirect methods for IgG and IgM detection and sandwich methods for antibodies detection.
HP811-08 NEW! Recombinant COVID-19 nucleocapsid antigen BA232 Suitable to use in ELISA, CLIA, CMIA and Rapid Test (POCT). Suggested indirect methods for IgG and IgM detection and sandwich methods for antibodies detection.

HP811-04 NEW!
Recombinant COVID-19 nucleocapsid antigen KA203 Suitable to use in ELISA, CLIA, CMIA and Rapid Test (POCT). Suggested indirect methods for IgG and IgM detection and sandwich methods for antibodies detection.
HA811-12H NEW! Recombinant humanized anti-covid -19 Nucleocapsid protein IgG positive control Suitable to use in ELISA, CLIA, CMIA and Rapid Test (POCT). The product is intended for in vitro diagnostic use in the quality control of serum on clinical chemistry and immunoassay systems.
LA557 Coronavirus (CoV-NP 229E) Antigen, Recombinant >95% ELISA, IgG/IgM detection Peptide composed of 359 amino acids containing immunodominant regions.
LA570 Coronavirus (SARS-CoV ENV; 1-76) Antigen, Recombinant >90% ELISA, IgG/IgM detection Peptide composed of amino acids 1-76 of envelope protein.
LA564L Coronavirus (SARS-CoV NP) Antigen, Recombinant >95% ELISA, WB Peptide composed of 422 amino acids of nucleoprotein. MW= 48 kDa
REC31807 Novel Coronavirus (2019-nCoV) Spike Glycoprotein (S2), Sheep Fc-Tag (HEK293) SDS-PAGE is a recombinant antigen which contains the Spike subunit 2 protein, amino acids 685-1211. Spike S2 is manufactured in mammalian HEK293 cells to ensure the most authentic post-translational modifications.
REC31806 Novel Coronavirus (2019-nCoV) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293) SDS-PAGE is a recombinant antigen which contains amino acids 1-674 of subunit 1. Spike S1 protein is manufactured in HEK293 mammalian cells, ensuring the most authentic post-translational modifications.

Detection Kits

PHOENIXDX® 2019-NCOV is a real-time RT-PCR-based detection system for the 2019 Wuhan coronavirus (2019-nCoV) with New FDA Approval. 2019-nCoV is considered a novel human coronavirus that is genetically distinct from the common human coronaviruses (229E, NL63, OC43, HKU1), which cause seasonal acute respiratory illness. It is also genetically distinct from the two newer human coronaviruses, MERS-CoV and SARS-CoV.

PHOENIXDX® 2019-NCOV detects the presence of 3 different and highly specific gene sequences of 2019-nCoV: E gene, N gene and RdRP gene. All 3 assays must be tested positive to confirm the sample as 2019-nCoV-positive.

Additionally, a non-infectious positive control and a negative human extraction control are included. The positive control is used to confirm functionality of the assays and overall PCR performance, the negative human extraction control is included to evaluate the quality of the RNA isolation independently from the 2019-nCoV assays.

Enquire now at Sales@2BScientific.com 

Diagnostic Kits

SKU Product Name Details
BZL-17043.T0010 CORONA Rapid Test IgG and IgM CE-IVD Immunological rapid test for the qualitative detection of IgG and IgM antibodies against SARS-CoV2
BZL-243001N-10 NADAL[R] COVID-19 IgG/IgM Test 10 test cassettes per package Detection of: COVID-19 IgG/IgM Rehearsals: Whole blood/serum/plasma, Qualitative determination From contents per package: 10 test cassettes, 10 pipettes, 1 buffer

ELISA Kits

Catalogue No. Name Suitability
RV-405200 Human Anti-SARS-CoV-2 Virus (COVID-19) Spike 1 IgG ELISA Kit an immunoassay suitable for quantifying IgG antibody activity specific for S1 subunit of the spike protein of the SARS-CoV-2 virus, etiologic agent for the COVID-19 respiratory disease, in serum or plasma of vaccinated, immunized and/or infected hosts.
AE-300700-1 RecombiVirus Mouse Hepatitis virus (MHV/Coronavirs) IgG ELISA Kit, 96 tests for detecting and quantifying IgG antibody activity specific for MHV spike glycoprotein (MVH-S) in serum or plasma.
RV-402300-1 Recombivirus Human Anti-Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike protein 2 (S2) antibody (IgG) ELISA kit, 96 tests, Quantitative an indirect ELISA suitable for quantifying IgG antibody activity for MERS-S2 protein in human serum, plasma or other qualified biological samples from vaccinated, immunized and/or infected hosts.
RV-402400-1 Recombivirus Human Anti-Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike protein 1 RBD (S1-RBD) antibody (IgG) ELISA kit, 96 tests, Quantitative An indirect ELISA suitable for quantifying IgG antibody activity for MERS Receptor-Binding Protein (RBD) in human serum, plasma or other qualified biological samples from vaccinated, immunized and/or infected hosts
ARG80959 Human ACE2 ELISA Kit ARG80959 Human ACE2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACE2 in serum, plasma, body fluids, tissue lysate or cell culture supernatant.
E-HD-C044 Human Corona Virus Disease 2019 (COVID-19) IgG/IgM Lateral Flow Assay Kit Is a rapid chromatographic immunoassay for the qualitative detection of IgG and IgM antibodies specific to COVID-19 in human whole blood, serum or plasma as an aid in the scientific research of primary and secondary COVID-19 infections

Proteins

Molecule Cat. No. Species Host Product Description
ACE2 protein AC2-H82E6 Human HEK293 Biotinylated Human ACE2 / ACEH Protein, His,Avitag™
AC2-H52H8 Human HEK293 Human ACE2 / ACEH Protein, His Tag
AC2-H82F9 Human HEK293 Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™
AC2-C52H7 Cynomolgus HEK293 Cynomolgus ACE2 / ACEH Protein, His Tag
AC2-R5246 Rat HEK293 Rat ACE2, His Tag
AC2-M5248 Mouse HEK293 Mouse ACE2, His Tag
S Protein SPN-C52H4 2019-nCoV HEK293 2019-nCoV S protein Full Length (R683A, R685A), His Tag
SPN-S52H5 SARS HEK293 SARS S protein Full Length (R667A), His Tag
S1 Protein S1N-C52H3 2019-nCoV HEK293 2019-nCoV S1 protein, His Tag
S1N-C82E8 2019-nCoV HEK293 Biotinylated 2019-nCoV S1 protein, His,Avitag™
S1N-S52H5 SARS HEK293 SARS S1 protein, His Tag
S Protein RBD SPD-C82E9 2019-nCoV HEK293 Biotinylated 2019-nCoV S protein RBD, His,Avitag™
SPD-C5255 2019-nCoV HEK293 2019-nCoV S protein RBD, Fc Tag
SPD-S52H6 SARS HEK293 SARS S protein RBD, His Tag
3CL-Mpro 3CO-C51H5 2019-nCoV E.coli 2019-nCoV 3CL-Mpro Protein, His Tag
Nucleocapsid Protein NUN-C51H9 2019-nCoV E.coli 2019-nCoV Envelope protein, His Tag
Envelope Protein ENN-C5128 2019-nCoV E.coli 2019-nCoV RNA-dependent RNA polymerase, His Tag
RNA-dependant RNA Polymerase RNE-C5143 2019-nCoV E.coli 2019-nCoV RNA-dependent RNA polymerase, His Tag

ReadyTag Antibodies

SKU Name Reported Applications Details
RT0269 ReadyTag anti-6-His WB, IP, IF Equivalent to anti FLAG) Clone: AP1501
RT0268 ReadyTag anti-HA ELISA, WB, IP, IF The 12CA5 mAb recognizes the 9-amino acid sequence YPYDVPDYA, derived from influenza virus hemagglutinin (HA). HA is commonly added to proteins of interest using recombinant DNA technology. The HA tag can then be used for detection or purification of the tagged protein.
RT0266 ReadyTag anti-6-His WB, IP, IF The 6-His mAb reacts with polyhistidine (6-His). Histidine tags are commonly added to proteins of interest using recombinant DNA technology. The histidine tag can then be used for detection or purification of the tagged protein.

Recombinant Antibodies

Catalogue Name Details Application
Ab01680-10.0 Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022] This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as COVID-19 Spike protein. NTRL, ELISA
Ab01680-15.0 Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022] This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" confromation of the spike protein (Joyce et al. 2020) This antibody has been attributed a potential to be developed as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of 2019-nCoV infections (Tian et al., 2020).
MHVS15-R-10 Recombinant (E. coli) Hepatitis virus (MHV/Coronavirs) Spike Protein S1 (>95% pure, His-tag, ~63 kda) MHV-S1 protein was expressed in E. Coli as histag fusion protein (extracellular domain, purity >95%, ~45 KDa). Purified protein is supplied in 50 mM Tris, pH 8, 0.25M NaCl, 5mM betamercaptoethanol,, 0.5mM ETDA, 0.25M imidzazole, and 8M Urea Western Blotting: load 100-200 ng/well. ELISA (50-100 ng antigen/well).
TGEVS15-R-10 Recombinant (E.coli) Transmissible Gastroenteritis Coronavirus (TGEV)-S1 protein (>95%) Recombinant TGEV-S1 was expressed in E.coli as a his-tag fusion protein (776 aa, >95%, ~84.9 kDa). Purified protein is supplied in 16.7mM Tris-HCl, 2M G-HCl, 0.17 mM EDTA, 1.7mM β-mercaptoethanol, 0.08M NaCl and 0.08M Imidazole ELISA, Western blot Cellular Activity, Histochemistry & Immunofluorescence
BCNP15-R-10 Recombinant (E.coli) Bovine Coronavirus (BCoV/BCV) nucleocapsid protein (GST-tag/His-tag, >95%) Recombinant Bovine Coronavirus nucleocapsid protein was expressed in E.coli as a his-tag fusion protein (448 aa, >95%, ~78 kda). Purified protein is supplied in 50mM Tris-HCl, 0.5 mM EDTA, 5mM βmercaptoethanol, 0.25M NaCl, 8M Urea, and 0.25M Imidazole ELISA, Western blot Cellular Activity, Histochemistry & Immunofluorescence

Small Molecules

SKU Name Primary Activity COVID-19 Rationale
10-1379 Hydroxychloroquine sulfate Autophagy inhibitor In clinical trials (alongside chloroquine) for SARS-CoV-2 infection
10-2473 Chloroquine phosphate Autophagy inhibitor Sigma 1 receptor binder (SARS-CoV-2 Nsp6 protein interacts with the Sigma receptor)
10-1009 Na Valproate HDAC inhibitor HDAC2 interacts with SARS-CoV-2 Nsp5
10-1062 Cycloheximide-N-ethylethanoate FKBP12 inhibitor mTORC1 pathway members interact with SARS-CoV-2 N and Orf8 proteins
10-1089 Nafamostat mesylate Serine protease inhibitor Targets viral cell entry via inhibition of ACE2
10-1103 FK-506 FKBP inhibitor mTORC1 pathway members interact with SARS-CoV-2 N and Orf8 proteins
10-1104 Rapamycin mTOR inhibitor mTORC1 pathway members interact with SARS-CoV-2 N and Orf8 proteins
10-1105 PKC-412 (Midostaurin) Protein kinase inhibitor (pan specific) Inhibits MARK2/3, which interact with SARS-CoV-2 Orf9b
10-1111 Verapamil L-type Ca2+ channel blocker Targets Multidrug resistance-associated protein 1 (MRP1), which interacts with SARS-CoV-2 Orf9c
10-1281 Ribavirin Antiviral agent Inhibits IMPDH2, which interacts with SARS-CoV-2 Nsp14
10-1311 MLN4924 (Pevonedistat) Nedd8 activating enzyme inhibitor Targets Cullin2, which interacts with SARS-CoV-2 Orf10
10-1361 JQ1 Bromodomain inhibitor SARS-CoV-2 transmembrane protein E binds to the bromodomain-containing proteins BRD2 and BRD4
10-1458 GBR-12909 Dopamine reuptake inhibitor Sigma receptor agonist (SARS-CoV-2 Nsp6 protein interacts with the Sigma receptor)
10-1569 Dabrafenib (GSK2118436) B-Raf mutant inhibitor Inibits NEK9, which interacts with SARS-CoV-2 Nsp9
10-2025 Entacapone COMT inhibitor COMT interacts with SARS-CoV-2 Nsp7
10-2057 Apicidin HDAC inhibitor HDAC2 interacts with SARS-CoV-2 Nsp5
10-2059 Ascomycin FKBP ligand mTORC1 pathway members interact with SARS-CoV-2 N and Orf8 proteins
10-2253 Captopril ACE inhibitor Targets viral cell entry via inhibition of ACE2

Viral RNA+DNA Preparation Kit

is designed for rapid and effective isolation of RNA and DNA from a variety of pathogen organisms such as virus or bacteria. Samples can be fresh or frozen plasma/blood (treated with anticoagulants excepted heparin), serum, other cell-free body fluids or pathogen-infected tissue.

The Kit is specifically designed to isolate high-quality nucleic acids using low elution volumes and allowing sensitive downstream analysis including quantitative PCR and RT-PCR. The purified RNA/DNA is free of proteins and nucleases. Viral RNA+DNA Preparation Kit uses lysis buffer including chaotropic salts to inactivate RNases/DNases and advanced silica-gel membrane technology for fast purification of intact RNA/DNA. The preparation procedure is optimized to give reproducible results within 30 min.

Also RNase-free Water is suitable to experiment and research of RNA. The RNase-free Water, non DEPC, was prepared from highly purified water by ultrafiltration without DEPC treatment. The product was confirmed to be free from Rnase and Dnase. 

Designing an Anti-Coronavirus Antibody ELISA?

If you need to prepare for mass production, what would be better than an ELISA plate blocker and stabilizer, which would save you time and resources.

WellChampion offers One blocking and stabilizing manufacturing step in 15 minutes.

As a customer says:
When using WellChampion, the coating, blocking and stabilization are all done in a single step, at room temperature, with no washes necessary. Based on my experience, when using this reagent in accordance to its manufacturer’s recommendations, one can expect a higher signal-to-noise ratio, a high reproducibility, in addition to an exceptionally fast and long-lasting coating. The only downside is that colleagues from other laboratories might ask for it.

Enquire at Info@2BScientific.com for further information

As a thank you for all our Research Colleagues doing incredible work in such times we would like to send a Covi-Shield with every order free of charge!

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