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Human Cytokine Products

Top Cytokine Products!

Highly Active Human Interferon Proteins

High quality recombinant proteins derived from bacterial or mammalian cells

  • Consistent performance and biological activity
  • 95% or greater purity
  • Endotoxin level < 1 EU/μg
  • Suitable for use in a range of applications including ELISAs & cytopathic effect assays

Featured:
Universal Type I Interferon (Cat. No. 11200)
Unique hybrid interferon exhibiting activity across multiple species

  • Useful for cross-species comparative studies of Type I IFN effects
  • Broad crossover to multiple animal models in vivo and in vitro where the species relevant Type I IFN-α is unavailable or performs inconsistently
  • High specific activity on multiple animal cell lines


Figure 1. Comparison of the Activities of Universal Type I IFN, Human IFN Alpha 2a, and Mouse IFN Alpha A

Cat. No. Description Size
11002-1 Human IFN Alpha (IFN-α) Sampler Set (subtypes also sold individually)* 1 x 10^5 U
11101-1, 2 Human IFN Alpha A (Alpha 2a), carrier-free 5 μg, 20 μg
11105-1 Human IFN Alpha 2 (Alpha 2b) 1 x 10^5 U
11175-1 Human IFN Alpha 1 (Alpha D) 1 x 10^5 U
11177-1 Human IFN Alpha 4a (Alpha M1) 1 x 10^5 U
11200-1, 2 Universal Type I IFN 1 x 10^5 U, 1 x 10^6 U
11395-1 Human IFN Omega 25 μg
11410-2 Human IFN Beta 1a, mammalian, carrier-free 1 x 10^6 U
11415-1 Human IFN Beta 1a, mammalian 1 x 10^5 U
11420-1 Human IFN Beta 1b, E.coli 1 x 10^5 U
11500-1, 2 Human IFN Gamma 50 μg, 100 μg
11720-1 Human IL-28A/IFN Lambda 2, HCE 1 x 10^5 U
11725-1 Human IL-29/IFN Lambda 1, HCE 1 x 10^5 U
11730-1 Human IL-28B/IFN Lambda 3, HCE 1 x 10^5 U
11821-1 Human IL-28A/IFN Lambda 2, carrier-free 25 μg
11826-1 Human IL-29/IFN Lambda 1, carrier-free 25 μg

*IFNa-A (2a); IFNa-B2 (8); IFNa-C (10); IFNa-D (1 [Val(114)]); IFNa-F (21); IFNa-G (5); IFNa-H2 (14); IFNa-I (17); IFNa-J1 (7); IFNa-K (6); IFNa-4b (4); IFNa-WA (16)

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Human Interferon Alpha (IFN-α) ELISAs

Featured:
Human IFN Alpha All Subtype ELISA (Cat. No. 41115)
High Sensitivity

  • Assay Standard Range: 1.95 – 125 pg/ml
  • Accurate low pg/ml detection of all human IFN-α subtypes
  • Robust matrix tolerance including autoimmune disease sera, normal serum, and tissue culture media (TCM)
  • Optimized for autoimmune serum and plasma samples
  • Consistent, reproducible results with < 10% inter- and < 8% intra-assay CVs
  • Detects previously “missed” IFN-α subtypes
Subtype LLOQ (pg/ml) LLOD (pg/ml)
α1 (αD) 2.31 0.76
α2a (αA) 1.36 0.42
α4a (αM1) 1.38 0.43
α5 (αG) 1.70 0.51
α6 (αK) 1.13 0.33
α7 (αJ1) 2.09 0.59
α8 (αB2) 2.81 0.82
α10 (αC) 1.77 0.65
α14 (αH) 1.76 0.51
α16 (αWA) 2.01 0.68
α17 (αI) 1.36 0.50
α21 (αF) 6.18 1.67

Table 1. Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) in Normal Human Serum.


Figure 2. IFN-α Specific Signal in Lupus Patients. Lupus patient serum number. Purple bars represent non-specific signal, i.e., signal not blocked by AntiIFN-α PAb.

  41100 41105 41110 41115
● Good   ● ● Better   ● ● ● Best Hu-IFN-α Hu-IFN-α Multi-Subtype Hu-IFN-α MultiSubtype Serum Hu-IFN-α All
Global IFN-α subtype measurement in serum or plasma     ● ● ● ● ●
Global IFN-α subtype measurement in TCM   ● ● ● ● ● ● ●
Reproducible results with < 10% inter- & intra-assay CVs ● ● ● ● ● ● ● ● ● ● ● ●
Cat. No. Description Size
41100-1, 2 VeriKine Human IFN Alpha ELISA Kit (TCM) 1 x 96-well plate, 5 x 96-well plate
41105-1, 2 VeriKine Human IFN Alpha Multi-Subtype ELISA Kit (TCM) 1 x 96-well plate, 5 x 96-well plate
41110-1, 2 VeriKine Human IFN Alpha Multi-Subtype Serum ELISA Kit (S, P, TCM) 1 x 96-well plate, 5 x 96-well plate
41115-1 VeriKine-HS Human IFN Alpha All Subtype ELISA Kit (S, P, TCM) 1 x 96-well plate

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Human Interferon Beta (IFN-β) ELISAs

Featured:
Human IFN Beta Serum ELISA (Cat. No. 41415)
High Sensitivity

  • Assay Standard Range: 1.2 – 150 pg/ml
  • Provides > 90% serum spike-recovery
  • Reproducible results with < 8% inter- and intra-assay CVs
  • Robust matrix tolerance for autoimmune disease sera
  • Validated by multiple CROs for sensitive measurement of IFN-β therapeutics in human serum samples
  • e.g. Rebif®, Avonex®, Extavia®, Betaseron®
  • Suitable for PK, PD, Toxicity & Biomarker studies


Figure 3. ELISA Standard Curve in Normal Human Serum (NHS) vs. Standard Curve in Standard Diluent. No observable inhibitory effect of normal human serum.


Figure 4. Multiple Sclerosis (MS). IFN-β was measured in sera/plasma of Multiple Sclerosis (MS) patients and Normal Donors. IFN-β was found to be quantifiable in 86% of MS patients on IFN-β therapy, 4.1% of MS patients on other therapies, and 1.5% of Normal Donor samples.

  41410 41415
● Good   ● ● Better   ● ● ● Best Hu-IFN-β Hu-IFN-β Serum
Accurate IFN-β measurement in serum or plasma (including autoimmune disease sera)   ● ● ●
Accurate IFN-β measurement in tissue culture media (TCM) ● ● ● ● ●
Reproducible results with < 10% inter- & intra-assay CVs ● ● ● ● ●
Cat. No. Description Size
41410-1, 2 VeriKine Human IFN Beta ELISA Kit (TCM) 1 x 96-well plate, 5 x 96-well plate
41415-1 VeriKine-HS Human IFN Beta Serum ELISA Kit (S, P, TCM) 1 x 96-well plate

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Anti-Human IFN Monoclonal (MAb) & Polyclonal (PAb) Antibodies

Labeled & unlabeled antibodies for a variety of uses

  • MAbs offer high specificity in applications where specific binding is desirable
  • PAbs are preferable for neutralization because they target a variety of specific epitopes of a particular antigen

Featured: Anti-Human IFNAR2, Clone MMHAR-2 (MAb) (Cat. No. 21385-1)

  • Blocks biological activity of human Type I IFNs and IFN-α receptor
  • Binds to human IFN-α receptor with high affinity

Human Type I IFN Neutralizing Antibody Mixture (Cat. No. 39000)
Mixture of MAbs and PAbs directed against human Type I IFNs & IFNAR2

  • Effectively neutralizes biological activity of human IFN-α, IFN-β, IFN-ω, IFN-κ, and IFN-ε
  • Improves neutralization of multiple Type I IFNs often found in complex samples by employing a broadly neutralizing custom antibody cocktail


Figure 5. Mouse MAb to Human IFN-Alpha/Beta Receptor 2.


Figure 6. Representative neutralization curves (diluted 1:50) to human IFN-α, -β, and -ω. A549 cells incbated with antibody mixture and titrated IFN, subsequently challenged with EMCV.

Cat. No. Description Size
Anti-Human IFN Alpha (MAb) (multiple clones available in varying sizes)
21112-3 FITC Conjugated Anti-Human IFN Alpha, Clone MMHA-11 (MAb) 25 μg
21370-1 Mouse MAb against Human IFN Alpha/Beta Receptor 1, Clone MMHAR-3 (MAb) 50 μg
21370-3 R-Phycoerythrin (PE) Conjguated Mouse MAb against Human IFNAR1, Clone MMHAR-3 (MAb) 50 tests
21375-1 Anti-Human IFN Alpha/Beta Receptor 1, Clone MMHAR-1 (MAb) 50 μg
21375-3 PE Conjugated Anti-Human IFN Alpha/Beta Receptor 1, Clone MMHAR-1 (MAb) 100 tests
21376-3 CFS Conjugated Anti-Human IFN Alpha/Beta Receptor 1, Clone MMHAR-1 (MAb) 100 tests
21385-1 Anti-Human IFN Alpha/Beta Receptor Chain 2 (IFNAR2), Clone MMHAR-2 (MAb) 50 μg
21385-3 PE Conjugated Anti-Human IFN Alpha/Beta Receptor Chain 2 (IFNAR2), Clone MMHAR-2 (MAb) 50 tests
Anti-Human IFN Beta (MAb) (multiple clones available in varying sizes)
21400-3 FITC Conjugated Anti-Human IFN Beta, Clone MMHB-3 (MAb) 20 μg
Anti-Human IFN Gamma (MAb) (multiple clones available in varying sizes)
21585-1 Anti-Human IFN Gamma Receptor Chain 2, Clone MMHGR-2 (MAb) 250 μg
21720-1 Anti-Human IL-28A/IFN Lambda 2, neutralizing, Clone MMHL-2 (MAb) 200 μg
21730-1 Anti-Human IL-28B/IFN Lambda 3, neutralizing, Clone MMHL-3 (MAb) 100 μg
21885-1 Anti-Human IFN Lambda Receptor 1, Clone MMHLR-1 (MAb) 50 μg
21900-1 Anti-Human ISG15, Clone 2.1 (MAb) 100 μg
Anti-Human IFN Alpha (PAb) (multiple available)
Anti-Human IFN Beta (PAb) (multiple available, including protein A or affinity purified)
31500-1 Anti-Human IFN Gamma, Rabbit Serum (PAb) 2 x 10^4 units
39000-1 Human Type I IFN Neutralizing Ab Mixture 0.2 ml

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!