FLEXIQuant Protein Expression Kit

Wheat Germ Cell-Free Expression System to Make Full-Length Isotope-Labeled MS Standards

Advantages

Premixed reagents for easy reaction setup using high performance wheat germ extract WEPRO®9240 MS
WEPRO®9240 MS includes [13C, 15N]-L-Lys and [13C, 15N]-L-Arg suitable for working with trypsin digestion
Very high labeling efficiency with incorporation rates of ~99%
FLEXIQuant vector provided to utilize FLEX-tag for absolute protein quantification
Option to use His-tag-mediated purification for protein MS standard
Use FLEX-tag and FLEX peptide standards for two-step quantification of target proteins

Prepare expression vector for FLEX-tagged protein of interest using vector provided with kit
Prepare isotope-labeled FLEX-tagged MS Standard using reagents provided with kit
Use Western Blotting to determine concentration range of MS Standard and target protein in sample
Add MS Standard directly sample before processing
To copurify and digest MS Standard together with proteins in extract
Add FLEX peptide provided with kit before peptide analysis by MS
Determine protein concentrations using protein MS Standard and FLEX peptide
Detailed protocol in the literature: A practical guide to the FLEXIQuant method. Singh S, Kirchner M, Steen JA, Steen H. Methods Mol Biol. 2012;893:295-319.

SKU	Product Name	Description	Reactions
CFS-FLEX-MS	FLEXIQuant Protein Expression Kit – MS Standards*	Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM Mass Spectrometry	8 rxns
		Kit provides vector EU-E01-TEV-N1-FLEX-tag
		Kit provides FLEX peptide
		Kit uses bilayer reaction format per reaction format on 226 μl scale
		One reaction can produce ~23 μg of crude CDC27 protein (positive control included in kit)
EDX-FLEX-PEP	FLEX-tag Peptide	Lyophilized, synthetic FLEX-tag peptide (TENLYFQGDISR); > 95% peptide purity, 1 nmol dried peptide per vial	8 vials
Related Products
EDX-PLUS-MS	Premium PLUS Expression Kit for MS	Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM Mass Spectrometry	16 reactions
		Kit uses bilayer reaction format on 226 μl scale
		One reaction can produce ~30 μg of crude DHFR protein (positive control included in kit)

CFS can provide all our expression vectors free of charge
Inquire about our protein expression services: CFS can prepare 13C/15N lysine/arginine labeled proteins for MRM/SRM Mass Spectrometry
Request reference list with examples on accurate quantitative analysis by FLEXIQuant methods