PD-L1

Programmed cell death protein 1 (PD-1), alternatively referred to as CD279, is an immunoglobulin superfamily member. It is expressed by activated T-cells and plays an important role in subsiding immune responses and promoting tolerance to self-antigens by suppressing T-cell activity. PD-1 has two natural ligands, known as PD-L1 (also known as CD274) and PD-L2 (CD273), that are mainly expressed by antigen presenting cells. Since over-expression of PD-L1 is common in many tumours, several monoclonal antibody therapeutics have been developed to block the PD-1/PD-L1 interaction. These include nivolumab (OPDIVO®) and pembrolizumab (Keytruda®), which have seen clinical success in treating melanoma.

PD-L1 related antibodies from preferred suppliers:

• BE0101 - InVivoMAb anti-mouse PD-L1 (B7-H1) - The 10F.9G2 (clone) monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274.
• BE0285 - InVivoMAb anti-human PD-L1 (B7-H1) - The 29E.2A3 monoclonal antibody reacts with human PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274.
• ARG65862 - anti-CD274 / PD-L1 antibody [SQab1716] - Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
• ARG11039 - anti-CD274 / PD-L1 antibody [12B4] 

More purified versions of the PD-L1 antibodies:

for more information on the difference between InVivoMAb and InVivoPlus please click HERE!

• BP0101 - InVivoPlus anti-mouse PD-L1 (B7-H1) - The 10F.9G2 monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274.

• ARG22566 - anti-CD274 / PD-L1 antibody [MIH6] (low endotoxin) - Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

PD-L1 related proteins from preferred suppliers:

• PD1-C5253 - Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag -  is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus. His Tag is also available.

• PD1-M5251 - Mouse PD-L1 / B7-H1 Protein, Fc Tag -  is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). His Tag is also available.

• PDL-H5250 - Human PD-L1 / B7-H1 Protein, Llama IgG2b Fc Tag, low endotoxin - is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1).

• PD1-H5258 - Human PD-L1 / B7-H1 Protein, Fc Tag (HPLC-verified) - is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

PD-L1 related conjugated proteins:

• PD1-M82F5 - Biotinylated Mouse PD-L1 / B7-H1, Fc Tag, Avi Tag (Avitag™) - is expressed from human HEK293 cells. It contains AA Phe 19 - Thr 238 (Accession # NP_068693).

• PD1-H82F3 - Biotinylated Human PD-L1 / B7-H1 Protein, Fc,Avitag™,His Tag - is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

• PD1-H82E5 - Biotinylated Human PD-L1 / B7-H1 Protein, Avitag™,His Tag (recommended for biopanning) -  is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

• PDL-H82F2 - Biotinylated Human PD-L1 / B7-H1 Protein, Fc,Avitag™ -  is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1).

PD-L1 related ELISA kits from preferred suppliers:

• EP-101 - PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair - This pair is useful for screening for inhibitors of human PD-1 binding to human PD-L1.

• ARG81929 - Human CD274 / PD-L1 ELISA Kit -  is an Enzyme Immunoassay kit for the quantification of human CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants. Results of a typical standard run with optical density reading at 450 nm.

• ARG81930 - Mouse CD274 / PD-L1 ELISA Kit - is an Enzyme Immunoassay kit for the quantification of human CD274 / PD-L1 in serum, plasma (heparin, EDTA) and cell culture supernatants. Results of a typical standard run with optical density reading at 450 nm.

PD-L1 related Cell Lines from preferred supplier:

- M00567 - CHO-K1/mouse PD-L1 - (Academic Discount Available) High protein expression at the cell surface validated by FACS. Stable cell lines for increased reliability and reproducibility (over 15 passages). Ready to use hot targets, saving 3-5 months of drug discovery program. For Binding Assay.