The term cytokine is derived from the Greek words for cell (cyto) and movement (kinesis), and encompasses a broad diversity of cell signalling molecules that includes interleukins, interferons, chemokines, the tumour necrosis factor family and various growth factors. Cytokines act on their target cells by binding to specific cell surface receptors, resulting in signalling cascades which ultimately alter cellular function. Such as
- Immunity - by triggering mechanisms like Natural Killer cells or Macrophages to combat against any external agent. (Interleukin & Interferon)
- Inflammation – these cytokines are excreted from macrophages and T helper cells to bring about inflammation. (TNF-alpha & IL-1)
- Haematopoiesis – when new blood cells are produced, the cytokines due their work upon immature cells by raising the rate of their proliferation and maturation. (GM-CSF & IL-7)
Cytokines affect almost every known biological process, but when levels of these important cellular communication molecules are disrupted, health can suffer. Therapeutic targeting of cytokine pathways therefore offers hope for patients with conditions ranging from rheumatoid arthritis, multiple sclerosis and psoriasis, to cancer, systemic lupus erythematosus (SLE) and schizophrenia.
We offer a comprehensive range of cytokine reagents from our carefully selected portfolio of suppliers. These include antibodies, antigens and a wide selection of kits.
Our extensive range of anti-cytokine antibodies includes products from ImmunoReagents or Exbio. The ImmunoReagents product range includes monoclonal and polyclonal anti-cytokine antibodies raised in a variety of host species, in addition to secondary antibodies covering a broad spectrum of immunoglobulins, cross absorbed to provide excellent specificity. Exbio affords a comprehensive choice of flow cytometry validated monoclonal antibodies in various formats, including a wide selection of directly conjugated antibodies.
Our monoclonal antibodies from Mabtech have been carefully characterised, and this information has been used to configure optimal antibody pairs for ELISpot, FluoroSpot and ELISA detection of cytokines. During an ELISpot assay, cells are cultured on a surface coated with a cytokine-specific capture antibody, and any secreted cytokine is subsequently detected through the addition of a second cytokine-specific detection antibody. The FluoroSpot assay combines the sensitivity of the ELISpot method with the capacity to analyse the secretion of more than one cytokine from the same cell, through reliance on a fluorometric readout rather than the measurement of a precipitate.
Shenandoah Biotechnology specialises in the manufacture of recombinant proteins from E.coli, and offers superior quality recombinant cytokines. Many of these are highly compatible with the ImmunoReagents antibodies, and can be used in a wide variety of research applications.
We offer a diverse selection of ELISA kits from various suppliers, covering most cytokine targets and species. These include high quality products from Aviva Systems Biology, Biosensis, Cusabio Biotech, Elabscience and Genway Biotech, to name just a few.