Pooled Perturb-Seq CRISPR KO Libraries

Libraries of sgRNAs containing clonal barcodes adjacent to the gRNA sequences of humans. This library is meant to be used with CRISP-Seq screens (aka Perturb-Seq screens). Researchers can detect the clonal barcodes and the sgRNA effector as well as the clonal barcodes with RNA sequencing in cells transduced with these libraries. This is so that changes in gene expression can be correlated with the disruption of genes. Our company, 2BScientific ltd, can offer you two CRISPR knockout libraries: a human collection targeting 27 anti-cancer genes and a mouse collection targeting 27 mouse homologs. Approximately 20 sgRNAs targets each gene's functional domain.