Pooled CRISPR KO Libraries

At 2BScientific we have the Cellecta's world's first single-module human genome-wide CRISPR knockout library designed to be small and versatile (80,000 constructs) enough to provide convenient genome-wide cell viability assays with most standard human cell lines. As a result of the four sgRNAs targeting each gene, which were all designed based upon the criteria for effective guides described by Doench, et al., as well as the Cellecta’s-designed HEAT sgRNA tracrRNA structure which has proven to generate more robust results than the standard sgRNA design, the library has sufficient gene coverage to generate efficient results.