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Pooled CRISPR KO Libraries

At 2BScientific we have the Cellecta's world's first single-module human genome-wide CRISPR knockout library designed to be small and versatile (80,000 constructs) enough to provide convenient genome-wide cell viability assays with most standard human cell lines. As a result of the four sgRNAs targeting each gene, which were all designed based upon the criteria for effective guides described by Doench, et al., as well as the Cellecta’s-designed HEAT sgRNA tracrRNA structure which has proven to generate more robust results than the standard sgRNA design, the library has sufficient gene coverage to generate efficient results.

Diagram credit : Samreen Islam created through Biorender website

Porduct Catalog

Catalog No. Description
KOHGW-80K-P CRISPR Human Genome 80K Knockout Library (plasmid)
KOHGW-80K-V8 CRISPR Human Genome 80K Knockout Library (virus)
KOHGW-80K-V9 CRISPR Human Genome 80K Knockout Library (virus)
KOHGW-80K-B-P CRISPR Module B Human Genome 80K Knockout Library (virus, sgRNA do not overlap with KOHGW-80K-P)
KOHGW-80K-B-V8 CRISPR Module B Human Genome 80K Knockout-B Library (virus, sgRNA do not overlap with KOHGW-80K-P)
KOHGW-80K-B-V9 CRISPR Module B Human Genome 80K Knockout-B Library (virus, sgRNA do not overlap with KOHGW-80K-P)
KOHGW-EP-80K-P CRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (plasmid)
KOHGW-EP-80K-V8 CRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (virus)
KOHGW-EP-80K-V9 CRISPR Human Genome 80K Knockout Library in EF1-Puro Vector (virus)
KOHGW-M1-P CRISPR Human Genome Knockout Library, Module 1 (plasmid)
KOHGW-M1-V8 CRISPR Human Genome Knockout Library, Module 1 (virus)
KOHGW-M1-V9 CRISPR Human Genome Knockout Library, Module 1 (virus)
KOHGW-M123-P CRISPR Human Genome Knockout Library, Modules 1, 2, & 3 (plasmid)
KOHGW-M2-P CRISPR Human Genome Knockout Library, Module 2 (plasmid)
KOHGW-M2-V8 CRISPR Human Genome Knockout Library, Module 2 (virus)
KOHGW-M2-V9 CRISPR Human Genome Knockout Library, Module 2 (virus)
KOHGW-M3-P CRISPR Human Genome Knockout Library, Module 3 (plasmid)
KOHGW-M3-V8 CRISPR Human Genome Knockout Library, Module 3 (virus)
KOHGW-M3-V9 CRISPR Human Genome Knockout Library, Module 3 (virus)
KOMGW-80K-P CRISPR Mouse Genome 80K Knockout Library (plasmid)
KOMGW-80K-V8 CRISPR Mouse Genome 80K Knockout Library (virus)
KOMGW-80K-V9 CRISPR Mouse Genome 80K Knockout Library (virus)

To get a quote for the products or to place an order, please email Sales@2BScientific.com.