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Gene Editing and CRISPR Products

An overview to applications of Gene Editing and CRISPR Products 

Gene editing refers to a group of technologies that allow researchers to edit an organism's DNA. These technologies permit genetic material to be added, altered, or removed at specific locations in the genome (15). The development of engineered nucleases, such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), which cause double-strand breaks (DSBs), has been a fundamental advance. DSBs play a significant role in site-specific mutagenesis since they stimulate the cell's DNA-repair mechanisms, such as homologous recombination and non-homologous end-joining (NHEJ). Procedures such as these use established, well-proven methodologies to use precise genetic edits, giving rise to cell lines with target gene deletions, integrations, or modifications (16). Despite these advantages, clustered regularly interspaced short palindromic repeats (CRISPR) offers many advantages over ZFNs and TALENs, such as simplicity of design and easy prediction of off-target sites. Also, it is possible to modify several genomic sites at one time (multiplexing). Therefore, CRISPR technology has revolutionised life science and is more accurate, cheaper, and faster than other genome-editing methods. The versatility and ease of use of CRISPR has transformed genome editing both for fundamental science and translational medicine.

This Prokaryote-derived CRISPR-Cas genome editing systems allow specific genetic manipulation of your genes by using sgRNA (single guide RNA, or gRNA) and Cas enzymes. CRISPR technique enables researchers to locate a specific DNA frame within a cell. In gene editing with CRISPR, the next step involves altering that piece of DNA. Furthermore, CRISPR has been adapted to do other tasks, such as turning genes on or off without altering their sequence (15,16,17).

At 2BScietific Ltd, our portfolio includes a wide variety of CRISPR/Cas9-based genome engineering products and services, including diverse lentiviral sgRNA constructs, siRNAs, Cas9 proteins, vectors, lentiviral pooled libraries, NGS read-out technologies, knockout cell lines, and screening services. 

To get access to our CRISPR associated and genomic analysis Products and Services, please contact us at Sales@2BScientific.com so that you can identify and select the best products for your experiments. Our experienced team is available to help you with application questions and product selection for your experiments.

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