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Buffers & General Consumables

VECTASHIELD[R] Vibrance[TM] Antifade Mounting Medium

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About this Product
Additional Names:
Mounting Media [Mounting Media ]
Extra Details:

VECTASHIELD[R] Vibrance[TM] Antifade Mounting Media are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.

Why VECTASHIELD Vibrance Antifade Mounting Media?

  • Superior antifade/anti-photobleaching properties across the spectrum prolongs possible imaging time
  • Compatibility with commonly used fluorophores
  • View sections one hour after mounting
  • No tone or autofluorescent background, even after curing
  • Room temperature storage of slides with extended archiving time
  • Minimal bubble formation, even after several weeks storage
  • Curing formulations with choice of counterstain (DAPI) or no counterstain
  • 12 month product expiration date

Plus the same features you have come to expect of the VECTASHIELD[R] brand:

  • Ideal refractive index
  • Easy to use
  • Ready to use, requires no warming
  • No sealing of coverslips required (curing formulations)
Shipping Conditions:
Storage Conditions:
Vector Laboratories
How quickly can I view sections after mounting with VECTASHIELD Vibrance (catalog #H-1700)? Is it recommended to wait one hour?:
Sections can be viewed immediately after mounting with VECTASHIELD® Vibrance, but one hour is recommended for optimal signal intensity.
How long does it take for the VECTASHIELD Vibrance to cure?:
With adequate removal of excess buffer, the VECTASHIELD Vibrance will cure enough by one hour to hold the coverslip in place. Complete curing takes about 4 – 24 hours at room temperature.
How long can I store slides mounted with the VECTASHIELD Vibrance?:
After mounting with VECTASHIELD Vibrance, slides can be stored at room temperature for several months without media retraction, bubble formation, or loss of signal intensity. We have not seen any difference between room temperature or storage at 4 degrees C.
Does VECTASHIELD contain glycerol?:
All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.
Do I need to dehydrate the tissue sections before applying VECTASHIELD?:
No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.
Do I need to seal the perimeter of the coverslip after I apply VECTASHIELD?:
That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers, no sealing of the coverslip is required.