VECTASHIELD[R] Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.
VECTASHIELD[R] Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes and will prolong possible imaging time.
Features: Inhibits photobleaching of dyes and fluorescent proteinsIdeal refractive indexReady to useNo warming necessaryOffered with nuclear or cytoskeletal counterstainsAvailable in non-hardening or hardening formulationsCan be stored without sealing for long term analysisEasy to use
The original VECTASHIELD[R] Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 Â°C, protected from light.
VECTASHIELD[R] HardSet[TM] Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
VECTASHIELD[R] Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please contact 2BScientific.com technical services to determine if VECTASHIELD[R] will be compatible in your system if you have any queries.
The original VECTASHIELD[R] Mounting Medium is available with or without the counterstain PI (propidium iodide). PI is a reddish nuclear counterstain with a broad excitation range at around 530 nm and emission at about 615 nm.
R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
The refractive index for VECTASHIELD[R] Mounting Medium is 1.45.