VECTASHIELD[R] Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.
VECTASHIELD[R] Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes and will prolong possible imaging time.
Features:Inhibits photobleaching of dyes and fluorescent proteinsIdeal refractive indexReady to useOffered with nuclear or cytoskeletal counterstainsAvailable in non-hardening or hardening formulationsNo warming necessaryCan be stored without sealing for long term analysisEasy to use
The original VECTASHIELD[R] Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.
VECTASHIELD[R] Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please contact 2BScientific.com technical services to determine if VECTASHIELD[R] will be compatible in your system if you have any queries.
The refractive index for VECTASHIELD[R] Mounting Medium is 1.45.
VECTASHIELD[R] Mounting Medium in Super Resolution Microscopy
The optimal medium for super-resolution imaging methods maximizes the lifetime and photo-switching characteristics of fluorophores. VECTASHIELD[R] Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIMM imaging, such as Cy5 or Alexa fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899).