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Buffers & General Consumables

VECTASHIELD[R] Antifade Mounting Medium

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About this Product
Additional Names:
Mounting Media [Mounting Media ]
Extra Details:
VECTASHIELD[R] Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.

VECTASHIELD[R] Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes and will prolong possible imaging time.


  • Inhibits photobleaching of dyes and fluorescent proteins
  • Ideal refractive index
  • Ready to use
  • Offered with nuclear or cytoskeletal counterstains
  • Available in non-hardening or hardening formulations
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Easy to use
  • The original VECTASHIELD[R] Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.

    VECTASHIELD[R] Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please contact technical services to determine if VECTASHIELD[R] will be compatible in your system if you have any queries.

    The refractive index for VECTASHIELD[R] Mounting Medium is 1.45.

    VECTASHIELD[R] Mounting Medium in Super Resolution Microscopy

    The optimal medium for super-resolution imaging methods maximizes the lifetime and photo-switching characteristics of fluorophores. VECTASHIELD[R] Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIMM imaging, such as Cy5 or Alexa fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899).

    Shipping Conditions:
    Storage Conditions:
    Vector Laboratories
    Can VECTASHIELD be used for super resolution microscopy?:
    Of the many variations of super resolution microscopy (SRM) currently being used, investigators have found the properties of product H-1000 (VECTASHIELD), to be advantageous in stochastic optical reconstruction microscopy (STORM) and structured illumination microscopy (SIM). Refer to the following published references for further information: 1) Olivier, N., et al (2013) Simple buffers for 3D STORM microscopy. Biochemical Optics Express 4:885-899. 2) Wegel., et al (2016) Imaging cellular structuires in super-resolution with SIM, STED and localisation microscopy: A practical comparison. Scientific Reports 6:27290.
    Does VECTASHIELD contain glycerol?:
    All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.
    Do I need to dehydrate the tissue sections before applying VECTASHIELD?:
    No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.
    Do I need to seal the perimeter of the coverslip after VECTASHIELD?:
    That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers, no sealing of the coverslip is required.