Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated
Product Sizes
Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated
£ POA
B-1375
About this Product
- SKU:
- B-1375
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Daffodil Lectin (DL), Alpha linked Mannose binding lectin
- Application:
- Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT
- Conjugate:
- Biotin
- Extra Details:
- This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers. NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting Sugar: 400 mM α-methylmannoside
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C
- Supplier:
- Vector Laboratories
- Manufacturer's Data Sheet:biotinylated-narcissus-pseudonarcissus