Pisum Sativum Agglutinin (PSA), Biotinylated
Product Sizes
Pisum Sativum Agglutinin (PSA), Biotinylated
£ POA
B-1055
About this Product
- SKU:
- B-1055
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Pea Lectin, Mannose binding Lectin
- Application:
- Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT
- Conjugate:
- Biotin
- Extra Details:
- Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.Biotinylated Pisum sativum agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C; Store frozen for long term storage
- Supplier:
- Vector Laboratories
- Manufacturer's Data Sheet:biotinylated-pisum-sativum-agglutinin-psa