Skip to content

Basket

You currently have no items in your basket.

Lens Culinaris Agglutinin (LCA), Biotinylated

SKU:
B-1045-5
Additional Names:
Lectins & Glycobiology Reagents [Lectins ], Lentil Lectin, Mannose binding Lectin
Application:
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT, FLUOROSPOT
Conjugate:
Biotin
Extra Details:
Lens culinaris agglutinin is composed of four subunits - two of about 17kDa and two of 8kDa. LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.Biotinylated Lens culinaris agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
Purification:
Affinity Purified
Research Area:
Glycobiology
Shipping Conditions:
Ambient
Storage Conditions:
2-8 [o]C; Store frozen for long term storage
Supplier:
Vector Laboratories
Size:
5mg
Price for 5mg £139.00

Academic Pricing

Please contact us for academic pricing.

Contact Us