Concanavalin A (Con A), Biotinylated
Product Sizes
5mg
£152.61
B-1005-5-5MG
About this Product
- SKU:
- B-1005-5
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Concanavalin enisformis (Jack Bean), alpha-methyl mannoside/ alpha- methylglucoside binding lectin
- Application:
- Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT
- Conjugate:
- Biotin
- Extra Details:
- Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C; Store frozen for long term storage
- Size:
- 5mg
- Supplier:
- Vector Laboratories
- Manufacturer's Data Sheet:biotinylated-concanavalin-a-con-a