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VECTASHIELD[R] HardSet[TM] Antifade Mounting Medium with DAPI

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VECTASHIELD[R] HardSet[TM] Antifade Mounting Medium with DAPI
Academic Pricing
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About this Product
Additional Names:
Mounting Media [Mounting Media ]
Extra Details:
VECTASHIELD[R] Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.

VECTASHIELD[R] Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes and will prolong possible imaging time.


  • Inhibits photobleaching of dyes and fluorescent proteins
  • Ideal refractive index
  • Ready to use
  • No warming necessary
  • Offered with nuclear or cytoskeletal counterstains
  • Available in non-hardening or hardening formulations
  • Can be stored without sealing for long term analysis
  • Easy to use
  • The original VECTASHIELD[R] Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.

    VECTASHIELD[R] HardSet[TM] Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

    VECTASHIELD[R] Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please contact technical services to determine if VECTASHIELD[R] will be compatible in your system if you have any queries.

    Both the VECTASHIELD[R] HardSet[TM] and the original VECTASHIELD[R] Mounting Media are available with or without the counterstain DAPI (4 , 6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD[R] Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

    Shipping Conditions:
    Storage Conditions:
    Vector Laboratories
    I am using VECTASHIELD HardSet Mounting Medium with DAPI (H-1500). The sections look good initially but bubble and vacuole formations occur under the coverslip. How can this be remedied?:
    Fixed, thin cut (<10 um), glass mounted sections are recommended to be used in combination with this medium. Unfixed material, thicker sections or the use of gasket or chamber slides or well formats may increase the incidence of bubble formation when applying H-1500. If using thin sections as described, ensure an adequate volume of the medium is being applied to spread out under the coverslip. Storage of slides in the freezer (-20 °C) may also reduce the incidence of bubbles. For existing mounted sections with bubble formation, coverslips can be removed by soaking the slides in buffer, and then remounted using fresh media.
    Does VECTASHIELD contain glycerol?:
    All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.
    Do I need to dehydrate the tissue sections before applying VECTASHIELD?:
    No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.
    Do I need to seal the perimeter of the coverslip after I apply VETASHIELD?:
    That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers, no sealing of the coverslip is required.
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