VECTASHIELD[R] Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.
VECTASHIELD[R] Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes and will prolong possible imaging time.
Features:Inhibits photobleaching of fluorescent dyes and fluorescent proteinsIdeal refractive indexReady to useOffered with nuclear or cytoskeletal counterstainsAvailable in non-hardening or hardening formulationsNo warming necessaryCan be stored without sealing for long term analysisEasy to use
The original VECTASHIELD[R] Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.
VECTASHIELD[R] HardSet[TM] Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.
VECTASHIELD[R] Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please contact 2BScientific.com technical services to determine if VECTASHIELD[R] will be compatible in your system if you have any queries.
Both the VECTASHIELD[R] HardSet[TM] and the original VECTASHIELD[R] Mounting Media are available with or without the counterstain DAPI (4 , 6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD[R] Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.