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Nitric Oxide Detection Kit

Academic Pricing:
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Additional Names:
Nitrogen monoxide; Nitrogen(II) oxide Detection Kit
Extra Details:
Nitric oxide (NO) is a diffusible, transient, reactive molecule that has physiological effects in the picomolar-to-micromolar range. Acting through soluble guanylate cyclase activation, NO is an important physiological regulator of the cardiovascular, nervous, and immunological systems (1). NO is bio-available by two routes. It can be endogenously generated by constitutive or induced enzymes like Nitric Oxide Synthase or it can be orally ingested as nitrates / nitrites for rapid uptake into circulation and subsequent conversion (2). The reactive nature of nitric oxide allows it to act as a cytotoxic factor when released during an immune response by cells such as macrophages. The reactivity also allows NO to be easily converted to a toxic radical that can produce nitrosative damage to cells, organelles and molecules such as DNA. Nitrosaylation however can be a regulated post-translational modification in cell signaling (3). The balance and dynamics of the regulatory/damage facets of NO are major forces in mitochondrial signaling and dysfunction (4). NO is linked not only to coronary heart disease, endothelial dysfunctions, erectile dysfunction, and neurological disorders, but also diabetes, chronic periodontitis, autism, cancer, and assorted age-related diseases (5-9). The physical properties of Nitric Oxide make it challenging for direct detection methods. However, colorimetric methods can be applied to measure its stable break-down products nitrate (-NO3) and nitrite (-NO2) (10).
3.125 - 200 µM
2.63 µM in the Nitrite and 1.02 µM in the Total Nitric Oxide
Shipping Conditions:
Blue Ice
Storage Conditions:
StressMarq Biosciences Inc
2 x 96 well
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Price for 2 x 96 well £346.52

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