A-172 Whole Cell Lysate
Product Sizes
A-172 Whole Cell Lysate
£ POA
W09-001-GL5
About this Product
- SKU:
- W09-001-GL5
- Additional Names:
- A172 Lysate, Cell Lysate, A-172 lysate
- Application:
- ChIP, Immunoprecipitation, SDS-PAGE, Western Blot
- Buffer:
- 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
- Concentration:
- 4 mg/ml
- Physical State:
- Liquid (sterile filtered)
- Shipping Conditions:
- Dry Ice
- Specificity:
- A-172 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibit
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=W09-001-GL5