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Proteins and Peptides

A-172 Whole Cell Lysate

Product Sizes
A-172 Whole Cell Lysate
£ POA
W09-001-GL5
About this Product
SKU:
W09-001-GL5
Additional Names:
A172 Lysate, Cell Lysate, A-172 lysate
Application:
ChIP, Immunoprecipitation, SDS-PAGE, Western Blot
Buffer:
1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
Concentration:
4 mg/ml
Physical State:
Liquid (sterile filtered)
Shipping Conditions:
Dry Ice
Specificity:
A-172 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibit
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
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