A431 Whole Cell Lysate
Product Sizes
A431 Whole Cell Lysate
£ POA
W09-000-361
About this Product
- SKU:
- W09-000-361
- Additional Names:
- A431 Lysate, Cell Lysate, A423
- Application:
- SDS-PAGE, Western Blot
- Buffer:
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
- Concentration:
- 1 mg/ml
- Physical State:
- Liquid
- Shipping Conditions:
- Dry Ice
- Specificity:
- A423 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibito
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=W09-000-361