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Proteins and Peptides

CHO/K1 Whole Cell Lysate

Product Sizes
CHO/K1 Whole Cell Lysate
£ POA
W07-000-359
About this Product
SKU:
W07-000-359
Additional Names:
CHO/K1 Whole Cell Lysate, CHO K1 WCL, CHO-K1 lysate, CHOK1 lysate
Application:
SDS-PAGE, Western Blot
Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Concentration:
1 mg/ml
Physical State:
Liquid
Shipping Conditions:
Dry Ice
Specificity:
The cells were grown in Ham's F12 medium containing 2 mM L-Gln and 1.5 g/L sodium bicarbonate supplemented with 10% FBS (Fetal Bovine Serum). Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein in
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
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