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Human IFN-gamma/Granzyme B/TNF-alpha FluoroSpotPLUS kit

Academic Pricing:
Please contact us at for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
pre-coated plates
Gene Details:
3458, 3002, 7124
Granzyme B, IFNG, TNFA
Dillenbeck, T., et al. (2014). Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-gamma, IL-17A and IL-22 Responses. Cells. ( Grist, J. T., et al. (2018). Extracellular Lactate: A Novel Measure of T Cell Proliferation. Journal of immunology. ( Birrueta, G., et al. (2019). Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. ( Lund, G., et al. (2017). Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. ( Bieling, M., et al. (2018). Personalized adoptive immunotherapy for patients with EBV-associated tumors and complications: Evaluation of novel naturally processed and presented EBV-derived T-cell epitopes. Oncotarget. (
Shipping Conditions:
Price £894.00

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