Skip to content

Basket

You currently have no items in your basket.

Human IFN-gamma/Granzyme B/TNF-alpha FluoroSpotPLUS kit

SKU:
FSP-011009-2
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3458, 3002, 7124
Immunogen:
Granzyme B, IFNG, TNFA
Reactivities:
Human
References:
Dillenbeck, T., et al. (2014). Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-gamma, IL-17A and IL-22 Responses. Cells. (http://www.ncbi.nlm.nih.gov/pubmed/25437441) Grist, J. T., et al. (2018). Extracellular Lactate: A Novel Measure of T Cell Proliferation. Journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/29288205) Birrueta, G., et al. (2019). Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/30891032) Lund, G., et al. (2017). Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. (http://www.ncbi.nlm.nih.gov/pubmed/29121407) Bieling, M., et al. (2018). Personalized adoptive immunotherapy for patients with EBV-associated tumors and complications: Evaluation of novel naturally processed and presented EBV-derived T-cell epitopes. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29435138)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Price £894.00

We use cookies to give you the best experience of using this website. By continuing to use this site, you accept our use of cookies. Please read our Cookie Policy for more information.