Human IFN-gamma/Granzyme B/TNF-alpha FluoroSpotPLUS kit SKU: FSP-011009-2 Academic Pricing: Please contact us at info@2bscientific.com for specific academic pricing Additional Names: The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion. Application: FluoroSpot Format: pre-coated plates Gene Details: 3458, 3002, 7124 Immunogen: Granzyme B, IFNG, TNFA Reactivities: Human References: Dillenbeck, T., et al. (2014). Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-gamma, IL-17A and IL-22 Responses. Cells. (http://www.ncbi.nlm.nih.gov/pubmed/25437441) Grist, J. T., et al. (2018). Extracellular Lactate: A Novel Measure of T Cell Proliferation. Journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/29288205) Birrueta, G., et al. (2019). Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/30891032) Lund, G., et al. (2017). Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. (http://www.ncbi.nlm.nih.gov/pubmed/29121407) Bieling, M., et al. (2018). Personalized adoptive immunotherapy for patients with EBV-associated tumors and complications: Evaluation of novel naturally processed and presented EBV-derived T-cell epitopes. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29435138) Shipping Conditions: Ambient Supplier: Mabtech Price £894.00 Qty: Add to order Hi, 2B Team here, how can we make a difference? Chat