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Human IFN-gamma/Granzyme B/IL-2 FluoroSpotPLUS kit

SKU:
FSP-011002-10
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B/IL-2 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3458, 3002, 3558
Immunogen:
Granzyme B, IFNG, IL2
Reactivities:
Human
References:
Bieling, M., et al. (2018). Personalized adoptive immunotherapy for patients with EBV-associated tumors and complications: Evaluation of novel naturally processed and presented EBV-derived T-cell epitopes. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29435138) Mateus, J., et al. (2020). Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science. (http://www.ncbi.nlm.nih.gov/pubmed/32753554) Danielzik, T., et al. (2017). Sensitive detection of rare antigen-specific T cells directed against Wilms' tumor 1 by FluoroSpot assay. Leuk Lymphoma. (http://www.ncbi.nlm.nih.gov/pubmed/28573960) Lindestam Arlehamn, C. S., et al. (2020). alpha-Synuclein-specific T cell reactivity is associated with preclinical and early Parkinson's disease. Nat Commun. (http://www.ncbi.nlm.nih.gov/pubmed/32313102) Fostier, K., et al. (2018). Impact of lenalidomide maintenance on the immune environment of multiple myeloma patients with low tumor burden after autologous stem cell transplantation. Oncotarget. (http://www.ncbi.nlm.nih.gov/pubmed/29755666)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-011009-10
Price £3894.00

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