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Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

SKU:
FSP-0110-2
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3002, 3458
Immunogen:
IFNG, Granzyme B, Granzyme B, IFNG
Reactivities:
Human
References:
Uebele, J., et al. (2017). Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus. PLoS Pathog. (http://www.ncbi.nlm.nih.gov/pubmed/28542586) Liebowitz, D., et al. (2020). Efficacy, immunogenicity, and safety of an oral influenza vaccine: a placebo-controlled and active-controlled phase 2 human challenge study. Lancet Infect Dis. (http://www.ncbi.nlm.nih.gov/pubmed/31978354) Jackson, S. E., et al. (2017). HCMV Specific CD4+ T Cells are poly-functional and can respond to HCMV Infected Dendritic Cells in vitro. Journal of virology. (http://www.ncbi.nlm.nih.gov/pubmed/28053099) Carrasco Pro, S., et al. (2018). Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/29734356) Brunskole Hummel, I., et al. (2016). Characteristics of immune memory 10–15 years after primary hepatitis B vaccination. Vaccine. (http://www.ncbi.nlm.nih.gov/pubmed/26718687)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-011009-2
Price £706.00

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