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Human IFN-gamma/Granzyme B FluoroSpotPLUS kit

SKU:
FSP-0110-10
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/Granzyme B FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3002, 3458
Immunogen:
IFNG, Granzyme B, Granzyme B, IFNG
Reactivities:
Human
References:
Jackson, S. E., et al. (2017). Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/28694811) Carrasco Pro, S., et al. (2018). Microbiota epitope similarity either dampens or enhances the immunogenicity of disease-associated antigenic epitopes. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/29734356) Lund, G., et al. (2017). Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. (http://www.ncbi.nlm.nih.gov/pubmed/29121407) Grist, J. T., et al. (2018). Extracellular Lactate: A Novel Measure of T Cell Proliferation. Journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/29288205) Jelcic, I., et al. (2018). Memory B Cells Activate Brain-Homing, Autoreactive CD4(+) T Cells in Multiple Sclerosis. Cell. (http://www.ncbi.nlm.nih.gov/pubmed/30173916)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-011009-10
Price £3047.00

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