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Human IFN-gamma/TNF-alpha FluoroSpotPLUS kit

SKU:
FSP-0109-2
Academic Pricing:
Please contact us at info@2bscientific.com for specific academic pricing
Additional Names:
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN--gamma/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (monoclonal antibody (mAb) clone CD3-2) is supplied as a positive control for cytokine secretion.
Application:
FluoroSpot
Format:
pre-coated plates
Gene Details:
3458, 7124
Immunogen:
IFNG, TNFA
Reactivities:
Human
References:
Schultz, H. S., et al. (2018). The effect of acylation with fatty acids and other modifications on HLA class II:peptide binding and T cell stimulation for three model peptides. PLoS ONE. (http://www.ncbi.nlm.nih.gov/pubmed/29758051) Jackson, S. E., et al. (2017). Latent Cytomegalovirus (CMV) Infection Does Not Detrimentally Alter T Cell Responses in the Healthy Old, But Increased Latent CMV Carriage Is Related to Expanded CMV-Specific T Cells. Front Immunol. (http://www.ncbi.nlm.nih.gov/pubmed/28694811) Lund, G., et al. (2017). Strong and frequent T-cell responses to the minor allergen Phl p 12 in Spanish patients IgE-sensitized to Profilins. Allergy. (http://www.ncbi.nlm.nih.gov/pubmed/29121407) Das, A., et al. (2017). Adaptive from Innate: Human IFN-gamma(+)CD4(+) T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. Journal of immunology. (http://www.ncbi.nlm.nih.gov/pubmed/28754679) Brunskole Hummel, I., et al. (2016). Markers of Protection in Children and Adolescents Six to Fourteen Years After Primary Hepatitis B Vaccination in Real Life. The Pediatric Infectious Disease Journal. (http://www.ncbi.nlm.nih.gov/pubmed/26569192)
Shipping Conditions:
Ambient
Supplier:
Mabtech
Related Product 1:
FSP-010209-2
Price £706.00

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