HighYield T7 RNA Crosslinking Kit (4-Thio-UTP), Synthesis of 4-Thio-modified RNA
Product Sizes
15 reactions
£406.00
RNT-135-15REACTIONS
About this Product
- SKU:
- RNT-135
- Extra Details:
- HighYield T7 RNA Crosslinking Kit (4-Thio-UTP) is designed to produce randomly 4-Thio-modified RNA probes via in vitro transcription. Such probes are ideally suited for the detection of RNA-Protein interactions by photocrosslinking. The photoreactive Thio group triggers the formation of a covalent bond between the RNA and closely interacting proteins upon UV irradiation. 4-Thio-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 80 % 4-Thio-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of 4-Thio-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting 4-Thio-modified RNA probe can be used for photocrosslinking experiments using a 365 nm UV light. The kit contains sufficient reagents for 15 labeling reactions of 20 ul each (100 % 4-Thio-UTP substitution, 7.5 mM ATP, 7.5 mM GTP, 7.5 mM CTP,7.5 mM 4-Thio-UTP). A 20 ul reaction with 100 % substitution yields about 10 ug RNA, with 80 % substitution about 50 ug RNA after 30 min incubation (1 ug T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling