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Proteins and Peptides

Atto550-LPETGG

Product Sizes
Atto550-LPETGG
£176.00
FP-040
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
FP-040
Extra Details:
Atto550-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label Atto550.  Atto550-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein.  Atto550-LPETGG contains the Atto550 fluorescent label which is efficiently excited in the range 540 - 565 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.
Molecular Weight:
1148.6
Purity:
>95% by hplc
References:
<p>Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem.<strong> 9</strong>(5) 802 PMID: 18297670 </p><p>Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. <strong>50</strong> 5024 PMID: 21538739 </p><p>Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. <strong>8</strong>(9) 1800 PMID: 23989674</p>
Sequence:
Atto550-Leu-Pro-Glu-Thr-Gly-Gly-OH
Shipping Conditions:
Ambient
Storage Conditions:
Store dry, frozen and in the dark
Size:
0.1 mg
Supplier:
Isca Biochemicals
Solubility:
Soluble in water
Formula:
C60H76N9O14