Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.000
View basket & checkout
Proteins and Peptides

Atto488-LPETGG

Product Sizes
Atto488-LPETGG
£176.00
FP-010
Academic Pricing
Please contact us for academic pricing.
Contact Us
About this Product
SKU:
FP-010
Extra Details:
Atto488-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label Atto488. Atto488-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto488-LPETGG contains the Atto488 fluorescent label which is efficiently excited in the range 480 - 515 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.
Molecular Weight:
1144.6
Purity:
>95% by HPLC
References:
<p>Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem. <strong>9</strong>(5) 802 <a href="https://pubmed.ncbi.nlm.nih.gov/18297670/" target="_blank" rel="nofollow">PMID: 18297670</a></p><p>Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. <strong>50</strong> 5024 <a href="https://pubmed.ncbi.nlm.nih.gov/21538739/" target="_blank" rel="nofollow">PMID: 21538739</a></p><p>Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. <strong>8</strong>(9) 1800 <a href="https://pubmed.ncbi.nlm.nih.gov/23989674/" target="_blank" rel="nofollow">PMID: 23989674</a></p>
Sequence:
Atto488-Leu-Pro-Glu-Thr-Gly-Gly-OH
Shipping Conditions:
Ambient
Storage Conditions:
Storage desiccated, frozen and in the dark
Size:
0.1 mg
Supplier:
Isca Biochemicals
Solubility:
Soluble in water
Formula:
C49H62N9O19 S2