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Antibodies

Anti-C5a [Fab 100]

Product Size(s)
1 mg
£1876.00
Ab00992-10.3-BT
Academic Pricing
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About this Product
SKU:
Ab00992-10.3-BT
Additional Names:
Complement Component 5a; Complement C5; C3 and PZP-Like Alpha-2-Macroglobulin Domain Containing Protein 4; Complement Component 5; C5a Anaphylatoxin; Prepro-C5; CPAMD4; Anaphylatoxin C5a Analog; ECLZB; C5D; C5b
Application:
ELISA
Buffer:
PBS only.
Clone:
Fab 100
Clonality:
Recombinant Monoclonal
Extra Details:
This chimeric human antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Host:
Human
Immunogen:
A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning, Fabs reactive with rC5a were isolated.
Isotype:
IgG1
Protein Details:
P01031
Purification:
Purified
Reactivities:
Human
References:
8157971
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Supplier:
Absolute Antibody
Type:
Antibody: Recombinant Antibodies
Size:
1 mg
LC Format:
kappa
Heavy Chain Modification:
Fc Silent[TM]
Original Species Name:
Mouse
Original Format:
Fab