Microspheres usually consist of a spherical shell that is usually made of a biodegradable or resorbable plastic polymer, that has a very small diameter usually in the micrometer or nanometer range, and that is often filled with a substance (as a drug or antibody) for release as the shell is degraded.
New miniaturised, high throughput technologies for bioseparation, diagnostics, DNA sequencing, flow cytometry, drug discovery, proteomics and genomics are in many instances reliant on attachment of functional biological molecules to a microsphere support. The vast array of life sciences applications include: capture reagents for immunoassay (fluorescence, enzyme linked etc); surfaces for immunoprecipitation; diagnostic assays; fluorescence microscopy; flow/imaging cytometry; magnetic cell separation; molecular diagnostics; agglutination tests; nucleic acid separation and protein separation among others.
MagReSyn - Conventional microsphere technologies use solid or porous/cracked microspheres with binding of biological molecules limited to the surface, a key factor constraining performance and the development of new applications for microsphere technologies and products. We have developed a novel (patented) microsphere technology platform, comprising a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microspheres. This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. The binding capacity serves as a major performance contributor to the number of applications and versatility for end-user applications by and further enables miniaturization, increased sensitivity and reducing the cost of R&D. Citations
|NTA||For rapid affinity purification of 6x-histidine-tagged proteins|
|SAX||For rapid protein fractionation by ion exchange|
|TiO2||TiO2-functional microspheres for highly-specific phosphopeptide enrichment|
|Ti-IMAC||Magnetic microspheres with chelated Ti4+ metal ions for highly-specific phosphopeptide enrichment|
|Amine||Primary amine functional support for ligand adsorption or chemical modification|
|Carboxyl||For covalent ligand immobilisation via primary amine residues|
|Streptavidin||High capacity for capture of biotinylated molecules|
|Protein A||High capacity Protein A for affinity purification of antibodies and immunoprecipitation|
|Protein G||High capacity Protein G for affinity purification of antibodies and immunoprecipitation|
|Trypsin||For rapid, automated tryptic digestion of proteins for mass spectrometry sample preparation|
|Chymotrypsin||For rapid, automated chymotryptic digestion of proteins for mass spectrometry sample preparation|
Bangs Labs Flow Cytometry Beads - Bangs Laboratories, Inc. is a manufacturer of uniform polymer, silica and magnetic microsphere products for diagnostic, research and flow cytometry applications. We also manufacture many specialty products to support validation and QC programs for analytical instruments, including a broad portfolio of fluorescent, count, size and cell viability standards.
fluidMAG - nanoparticles are ferrofluids consisting of an aqueous dispersion of magnetic iron oxides with diameters of 50 nm, 100 nm and 200 nm. The particles are covered with hydrophilic polymers which protect them against aggregation by foreign ions. Terminal functional groups such as ion-exchange groups or reactive groups for covalent immobilization can be used for binding to biomolecules. Ferrofluids can further be used for MRI-diagnostics and magnetic drug targeting applications.