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ZytoPure® FISH Probes for Sarcomas

In situ hybridization

Zytomed Systems is proud to offer the new product line ZytoPure® FISH, a reliable and accurate tool for the detection of genetic aberrations in FFPE tissue sections, cell samples and metaphase spreads by fluorescence in situ hybridization. ZytoPure® FISH Probes display brilliant signals, low background, and an excellent signal to noise ratio. They are intended to be used in combination with the ZytoPure® FISH Accessory Kit comprising all reagents necessary for performing a FISH procedure on FFPE sections. The kit contains just 4 reagents and includes a robust and easy to follow protocol. All ZytoPure® FISH Probes as well as the ZytoPure® FISH Accessory Kit are CE/IVD-labelled.

Background information: ZytoPure® FISH Probes for sarcomas

Numerous recurrent genomic rearrangements have been described in a number of bone and soft tissue tumors in the last 15 years [1, 2]. To give just a few examples, rearrangement of the USP6 gene occurs in the majority of primary aneurysmal bone cysts [3], and also in nodular fasciitis [4]. SS18 rearrangement is highly specific for synovial sarcomas [5], and the majority of Ewing sarcomas is characterized by EWSR1 rearrangements [1]. Amplification of the MDM2 gene typically occurs in liposarcomas (but not lipomas) [6], and in certain osteosarcomas. In combination with histological and radiological findings, FISH-based detection of these rearrangements is a very important tool for the often complex differential diagnosis of these entities.

Product information

ZytoPure® FISH Probes for sarcomas

Description Labeling CE/IVD Volume Cat. No.
MDM2/CEP12 ZytoPure® FISH Probe green/orange X 20 μl F2C006-002
100 μl F2C006-010
USP6 Break Apart ZytoPure® FISH Probe green/orange X 20 μl F2C007-002
100 μl F2C007-010
EWSR1 Break Apart ZytoPure® FISH Probe orange/green X 20 μl F2C008-002
100 μl F2C008-010
SS18 Break Apart ZytoPure® FISH Probe orange/green X 20 μl F2C009-002
100 μl F2C009-010
FUS Break Apart ZytoPure® FISH Probe green/orange X 20 μl F2C010-002
100 μl F2C010-010

Ancillary Reagents

Description CE/IVD Volume Cat. No.
ZytoPure® FISH Accessory Kit
(Pepsin Solution, 4 ml; FISH Pretreatment Buffer, 500 ml;
20x FISH Wash Buffer, 2 x 50 ml; DAPI/Antifade Solution,
800 μl)
X 1 Kit (20 tests) FA-Kit1-20
Pepsin Solution X 4 ml FA-001-004
FISH Pretreatment Buffer (rtu) X 500 ml FA-002-500
20x FISH Wash Buffer X 50 ml FA-003-050
DAPI/Antifade Solution X 800 μl FA-004-008
Fixogum (Rubber Cement) - 50 g ZY-FX50
125 g ZY-FX125

Other ZytoPure® FISH Probes

Description Labeling CE/IVD Volume Cat. No.
ERBB2/CEP17 ZytoPure® FISH Probe orange/green X 20 μl F2C001-002
100 μl F2C001-010
ALK Break Apart ZytoPure® FISH Probe green/orange X 20 μl F2C002-002
100 μl F2C002-010
ROS1 Break Apart ZytoPure® FISH Probe orange/green X 20 μl F2C003-002
100 μl F2C003-010
RET Break Apart ZytoPure® FISH Probe orange/green X 20 μl F2C004-002
100 μl F2C004-010
MET/CEP7 ZytoPure® FISH Probe green/orange X 20 μl F2C005-002
100 μl F2C005-010

ZytoPure ® Fluorochromes

Fluorochrome Excitation Emission
PureGreen 498 nm 532 nm
PureOrange 550 nm 576 nm

Literature

[1] Puls F et al. Histopathol 64:461-476, 2014
[2] Vargas AC et al. Asia Pac J Clin Oncol 15:38-47, 2019
[3] Oliveira AM et al. Am J Pathol 165:1773-1780, 2004
[4] Erickson-Johnson MR et al. Lab Invest 91:1427-1433, 2011
[5] Tanas MR et al. Arch Pathol Lab Med 134:1797-1803, 2010
[6] Ware PL et al. Am J Clin Pathol 141:334-341, 2014

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