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Species on Species Detection

Cross reactivity of any detecting reagents with the sample species is always a concern. Where primary antibodies are raised in the same host as the sample to be tested this is a key concern.  

Vector Laboratories offer solutions for using Mouse primary antibodies on mouse tissue, and in 2020 released a kit enabling detection of human (or humanised) antibodies on human tissue.

Optimised Human on Human Immunodetection

Optimised Human on Human Immunodetection

The H.O.H. Immunodetection Kit can be applied to most cell and tissue based staining situations where the detection of endogenous human IgG would be problematic when using a human, humanized, or chimeric human primary antibody. This kit is effective in eliminating confounding interference from endogenous human IgG on highly antigenic frozen or paraffin embedded tissue sections.

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Troubleshooting Guide

Troubleshooting Guide

Localizing a mouse primary antibody on mouse tissue using an anti-mouse immunoglobulin (Ig) detection system can produce nonspecific staining due to the presence of endogenous mouse Ig

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M.O.M. ImmPRESS Kit

M.O.M. ImmPRESS Kit

For years, researchers attempting to localize mouse primary antibodies on mouse tissue have relied on the Vector M.O.M. Kit to produce crisp, strong, specific staining of antibody targets.

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