Reveal true immunofluorescence—even in the most challenging tissues
In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. The novel, patent-pending Vector®TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.
Why TrueVIEW™ Quencher?
- Specific reduction of autofluorescence from non-lipofuscin sources
- Easy-to-use, one-step method
- Quick 5 min incubation
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
- Fluorescence assays
- Vector TrueView kit contains VECTASHIELD Vibrance Mounting media for fluorescent signal preservation
- Quenches autofluorescence caused by aldehyde fixation, and on problem tissues such as liver, spleen, red blood cells & kidney.
WITHOUT TreatmentWITH With Vector®TrueVIEW™
Reduction of autofluorescence in human spleen using the TrueVIEW™ Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.
Easy to Use
Following completion of immunofluorescence staining:
Mode of Action
Following completion of the IF staining procedure:
Signal to Noise Optimization
The primary antibody and detection reagents should be optimized (titered) in conjunction with Vector TrueVIEW Quenching Kit to achieve maximum signal to noise.
Optimization of Exposure
Increasing exposure times may be necessary to achieve optimal image acquisition for Vector®TrueVIEW™ Quencher treated slides.
Comparison Between Vector® TrueVIEW™ Reagent and Other Autofluorescence Reducing Agents
We compared the effectiveness of Vector TrueVIEW quenching action in parallel with other commercially available autofluorescence reducing products and "home brew" reagents, on serial sections of formalin-fixed, paraffin-embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted. The images below highlight our results. All images were acquired under identical conditions (including microscope objective and exposure times).