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TrueVIEW

Dramatically Reduce Autofluorescence

Vector®TrueVIEW™ Autofluorescence Quenching Kit

Reveal true immunofluorescence—even in the most challenging tissues

In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. The novel, patent-pending Vector®TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunoflu

Why TrueVIEW™ Quencher?

  • Specific reduction of autofluorescence from non-lipofuscin sources
  • Easy-to-use, one-step method
  • Quick 5 min incubation
  • Compatible with a wide selection of fluorophores
  • Compatible with standard epifluorescence and confocal laser microscopes
  • Fluorescence assays

                   WITHOUT Treatment                              WITH Vector®TrueVIEW™

Reduction of autofluorescence in human spleen using the TrueVIEW™ Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.

Easy to Use

Following completion of immunofluorescence staining:

Mode of Action

Following completion of the IF staining procedure:

Signal to Noise Optimization

The primary antibody and detection reagents should be optimized (titered) in conjunction with Vector TrueVIEW Quenching Kit to achieve maximum signal to noise.

Optimization of Exposure

Increasing exposure times may be necessary to achieve optimal image acquisition for Vector®TrueVIEW™ Quencher treated slides.

Comparison Between Vector® TrueVIEW™ Reagent and Other Autofluorescence Reducing Agents

We compared the effectiveness of Vector TrueVIEW quenching action in parallel with other commercially available autofluorescence reducing products and "home brew" reagents, on serial sections of formalin-fixed, paraffin-embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted. The images below highlight our results. All images were acquired under identical conditions (including microscope objective and exposure times). 

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