Glysite™ Scout, Immunofluorescent Glycan Screening Kit - interrogate your tissue for glycan expression!
Changes in glycosylation are known to occur in a number of pathologies, but as yet are not fully understood or well catalogued. Like other post translational modifications, glycosylation of proteins can have a big impact on their function.
Glycan monitoring may be a useful tool that had not been considered, but glycans can be used to detect cell responses to treatment or stimulus, they can affect biomarker association, and they can also be used to differentate cell types in the tumour microenvironment.
Glysite™ Scout Glycan Screening Kits, Immunofluorescence, are fully integrated kits for the detection of glycan expression in tissue sections. This curated lectin selection enables detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen.
| Lectin |
Binding Motifs (1) |
Category |
| GNL, Biotinylated |
Terminal α1-3 or α1-6 mannose |
Mannose |
| PHA-L, Biotinylated |
β1-6 branched N-glycans, Binds tri- and tetraantennary |
Complex N-glycan |
| Jacalin, Biotinylated |
Core 1 and 3 O-glycans, 3-substituted GalNAcα |
Core O-glycan |
| AAL, Biotinylated |
αFuc |
Fucose |
| MAL II, Biotinylated |
α2-3-sialylated Galβ1-3GalNAc in O-glycans, 3' sulfated βGal |
Sialic Acid and Sulfation |
| WGA, Biotinylated |
Terminal GlcNAcβ, Terminal GlcNAcα, Terminal NAc containing glycans |
GlcNAc and chitin |
| ECL, ECA, Biotinylated |
Terminal type 2 LacNAc, Terminal type 2 LacdiNAc |
Gal and LacNAc |
| WFA, WFL, Biotinylated |
Terminal GalNAcβ, Terminal GalNAcα, Terminal multiantennary LacNAc |
GalNAc |
(1) Bohar D, et al. 2022. A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities. ACS Chemical Biology.
These kits contain all you need for the detection protocol, including Carbo-Free blocking reagent, streptavidin blocking reagent, biotinylated lectin panel, DyLight™ Streptavidin (488, 594 or 649) as well as VECTASHIELD® Vibrance Mounting Media. The protocol is around 150 minutes, and similar to using a biotinylated primary antibody, so you're likely familiar with it already!
